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. 1992 Oct;30(10):2662–2667. doi: 10.1128/jcm.30.10.2662-2667.1992

Purification, characterization, and seroactivity of a 20-kilodalton Brucella protein antigen.

M S Zygmunt 1, F B Gilbert 1, G Dubray 1
PMCID: PMC270495  PMID: 1400966

Abstract

An internal protein was purified from cell extracts of Brucella melitensis B115 by a combination of preparative isoelectric focusing and high-performance size exclusion chromatography. The protein has an apparent molecular mass of 230 kDa as determined by size exclusion chromatography. The protein was resolved to a single band of 20 kDa after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The native protein had an isoelectric point of 4.9. The N-terminal sequence of the 20-kDa protein was determined. The 20-kDa protein has been identified as antigen A-2 with a previously described anti-antigen A-2 serum (B. Stemshorn, K. Nielsen, and B. Samagh, Can. J. Comp. Med. 45:77-81, 1981). Antigen A-2 reacted with sera from infected sheep in immunoblotting and may be useful in developing diagnostic tests for brucellosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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