Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Oct;30(10):2680–2685. doi: 10.1128/jcm.30.10.2680-2685.1992

Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex.

P Gerner-Smidt 1
PMCID: PMC270498  PMID: 1383266

Abstract

The Acinetobacter calcoaceticus-Acinetobacter baumannii complex consists of four genotypically distinct but phenotypically very similar bacterial species or DNA groups: A. calcoaceticus (DNA group 1), A. baumannii (DNA group 2), unnamed DNA group 3 (P. J. M. Bouvet and P. A. D. Grimont, Int. J. Syst. Bacteriol. 36:228-240, 1986), and unnamed DNA group 13 (I. Tjernberg and J. Ursing, APMIS 97:595-605, 1989). Because strains in this complex cause nosocomial outbreaks, it is important to be able to identify them as completely as possible. Ribotyping could provide such identification. Therefore, ribotyping was done on 70 strains in the A. calcoaceticus-A. baumannii complex with known DNA group affiliations by use of restriction enzymes EcoRI, ClaI, and SalI. A nonradioactive digoxigenin-11-dUTP-labeled Escherichia coli rRNA-derived probe was used. With any of the three restriction enzymes, banding patterns that were specific for each DNA group were seen. All 70 strains showed banding patterns that could identify them to the correct DNA group by use of any two of the three enzymes. In addition, banding patterns that could separate strains within any one DNA group were present. The discriminatory index of P. Hunter and M. Gaston (J. Clin. Microbiol. 26:2465-2466, 1988), applied to all strains with the combined results obtained with all three enzymes, revealed a value of 0.99. For strains in each DNA group, the value varied from 0.93 to 0.98. These results indicate the high discriminatory power of the system when used for epidemiological typing.

Full text

PDF
2683

Images in this article

2683Image
on p.2683

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen K. D., Green H. T. Hospital outbreak of multi-resistant Acinetobacter anitratus: an airborne mode of spread? J Hosp Infect. 1987 Mar;9(2):110–119. doi: 10.1016/0195-6701(87)90048-x. [DOI] [PubMed] [Google Scholar]
  2. Beck-Sagué C. M., Jarvis W. R., Brook J. H., Culver D. H., Potts A., Gay E., Shotts B. W., Hill B., Anderson R. L., Weinstein M. P. Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am J Epidemiol. 1990 Oct;132(4):723–733. doi: 10.1093/oxfordjournals.aje.a115714. [DOI] [PubMed] [Google Scholar]
  3. Bouvet P. J., Jeanjean S., Vieu J. F., Dijkshoorn L. Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol. 1990 Feb;28(2):170–176. doi: 10.1128/jcm.28.2.170-176.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Castle M., Tenney J. H., Weinstein M. P., Eickhoff T. C. Outbreak of a multiply resistant Acinetobacter in a surgical intensive care unit: epidemiology and control. Heart Lung. 1978 Jul-Aug;7(4):641–644. [PubMed] [Google Scholar]
  5. Dijkshoorn L., Michel M. F., Degener J. E. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol. 1987 Jun;23(4):313–319. doi: 10.1099/00222615-23-4-313. [DOI] [PubMed] [Google Scholar]
  6. Fagon J. Y., Chastre J., Domart Y., Trouillet J. L., Pierre J., Darne C., Gibert C. Nosocomial pneumonia in patients receiving continuous mechanical ventilation. Prospective analysis of 52 episodes with use of a protected specimen brush and quantitative culture techniques. Am Rev Respir Dis. 1989 Apr;139(4):877–884. doi: 10.1164/ajrccm/139.4.877. [DOI] [PubMed] [Google Scholar]
  7. French G. L., Casewell M. W., Roncoroni A. J., Knight S., Phillips I. A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. J Hosp Infect. 1980 Jun;1(2):125–131. doi: 10.1016/0195-6701(80)90044-4. [DOI] [PubMed] [Google Scholar]
  8. Gerner-Smidt P. Endemic occurrence of Acinetobacter calcoaceticus biovar anitratus in an intensive care unit. J Hosp Infect. 1987 Nov;10(3):265–272. doi: 10.1016/0195-6701(87)90008-9. [DOI] [PubMed] [Google Scholar]
  9. Gerner-Smidt P., Hansen L., Knudsen A., Siboni K., Søgaard I. Epidemic spread of Acinetobacter calcoaceticus in a neurosurgical department analyzed by electronic data processing. J Hosp Infect. 1985 Jun;6(2):166–174. doi: 10.1016/s0195-6701(85)80094-3. [DOI] [PubMed] [Google Scholar]
  10. Gerner-Smidt P., Tjernberg I., Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol. 1991 Feb;29(2):277–282. doi: 10.1128/jcm.29.2.277-282.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Giammanco A., Vieu J. F., Bouvet P. J., Sarzana A., Sinatra A. A comparative assay of epidemiological markers for Acinetobacter strains isolated in a hospital. Zentralbl Bakteriol. 1989 Dec;272(2):231–241. doi: 10.1016/s0934-8840(89)80012-x. [DOI] [PubMed] [Google Scholar]
  12. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  13. Hartstein A. I., Rashad A. L., Liebler J. M., Actis L. A., Freeman J., Rourke J. W., Jr, Stibolt T. B., Tolmasky M. E., Ellis G. R., Crosa J. H. Multiple intensive care unit outbreak of Acinetobacter calcoaceticus subspecies anitratus respiratory infection and colonization associated with contaminated, reusable ventilator circuits and resuscitation bags. Am J Med. 1988 Nov;85(5):624–631. doi: 10.1016/s0002-9343(88)80233-x. [DOI] [PubMed] [Google Scholar]
  14. Hunter P. R., Gaston M. A. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol. 1988 Nov;26(11):2465–2466. doi: 10.1128/jcm.26.11.2465-2466.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Joly-Guillou M. L., Bergogne-Berezin E., Vieu J. F. A study of the relationships between antibiotic resistance phenotypes, phage-typing and biotyping of 117 clinical isolates of Acinetobacter spp. J Hosp Infect. 1990 Jul;16(1):49–58. doi: 10.1016/0195-6701(90)90048-s. [DOI] [PubMed] [Google Scholar]
  16. Mekalanos J. J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov;35(1):253–263. doi: 10.1016/0092-8674(83)90228-3. [DOI] [PubMed] [Google Scholar]
  17. Musser J. M., Kroll J. S., Granoff D. M., Moxon E. R., Brodeur B. R., Campos J., Dabernat H., Frederiksen W., Hamel J., Hammond G. Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis. 1990 Jan-Feb;12(1):75–111. doi: 10.1093/clinids/12.1.75. [DOI] [PubMed] [Google Scholar]
  18. Owen R. J. Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol. 1989 Oct;30(2):89–99. doi: 10.1099/00222615-30-2-89. [DOI] [PubMed] [Google Scholar]
  19. Sakata H., Fujita K., Maruyama S., Kakehashi H., Mori Y., Yoshioka H. Acinetobacter calcoaceticus biovar anitratus septicaemia in a neonatal intensive care unit: epidemiology and control. J Hosp Infect. 1989 Jul;14(1):15–22. doi: 10.1016/0195-6701(89)90129-1. [DOI] [PubMed] [Google Scholar]
  20. Saunders N. A., Harrison T. G., Kachwalla N., Taylor A. G. Identification of species of the genus Legionella using a cloned rRNA gene from Legionella pneumophila. J Gen Microbiol. 1988 Aug;134(8):2363–2374. doi: 10.1099/00221287-134-8-2363. [DOI] [PubMed] [Google Scholar]
  21. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  22. Tjernberg I., Ursing J. Clinical strains of Acinetobacter classified by DNA-DNA hybridization. APMIS. 1989 Jul;97(7):595–605. doi: 10.1111/j.1699-0463.1989.tb00449.x. [DOI] [PubMed] [Google Scholar]
  23. Ygout J. F., Housset B., Derenne J. P., Daguet G. L. Hospital-acquired Acinetobacter baumanii pneumonitis. Lancet. 1987 Apr 4;1(8536):802–802. doi: 10.1016/s0140-6736(87)92822-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES