Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Jul;18(1):63–70. doi: 10.1128/jcm.18.1.63-70.1983

Comparison of methods for quantitating antigen-specific immunoglobulin M antibody with a reverse enzyme-linked immunosorbent assay.

J P Siegel, J S Remington
PMCID: PMC270745  PMID: 6885993

Abstract

We compared two methods for quantitating antigen-specific antibody by a reverse enzyme-linked immunosorbent assay. In the serial-dilution method, the result was determined to be the highest dilution of a serum yielding an absorbance above an established threshold. In the single-dilution method, the result was determined by comparing the absorbance yielded by the test serum at a standard dilution to that yielded by positive and negative reference sera at the same dilution. The results in the single-dilution method reflected the antigen-specific immunoglobulin M antibody activity as a proportion of total immunoglobulin M antibody in a serum, i.e., the immune load, whereas the results in the serial-dilution method reflected the absolute concentration of antigen-specific immunoglobulin M antibody activity. Compared with results in the serial-dilution method, results in the single-dilution method had considerably greater reproducibility on a day-to-day basis and under various test conditions. The single-dilution method was more useful in discriminating between sera from patients in an early stage of clinical infection due to Toxoplasma gondii and sera from patients in a later stage of infection.

Full text

PDF
64

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araujo F. G., Remington J. S. Antigenemia in recently acquired acute toxoplasmosis. J Infect Dis. 1980 Feb;141(2):144–150. doi: 10.1093/infdis/141.2.144. [DOI] [PubMed] [Google Scholar]
  2. Duermeyer W., Wielaard F., van der Veen J. A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A. J Med Virol. 1979;4(1):25–32. doi: 10.1002/jmv.1890040104. [DOI] [PubMed] [Google Scholar]
  3. Duermeyer W., van der Veen J. Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet. 1978 Sep 23;2(8091):684–685. doi: 10.1016/s0140-6736(78)92802-7. [DOI] [PubMed] [Google Scholar]
  4. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  5. Fields H. A., Davis C. L., Dreesman G. R., Bradley D. W., Maynard J. E. Enzyme potentiated radioimmunoassay (EPRIA): a sensitive third-generation test for the detection of hepatitis B surface antigen. J Immunol Methods. 1981;47(2):145–159. doi: 10.1016/0022-1759(81)90115-0. [DOI] [PubMed] [Google Scholar]
  6. Gerlich W. H., Lüer W. Selective detection of IgM-antibody against core antigen of the hepatitis B virus by a modified enzyme immune assay. J Med Virol. 1979;4(3):227–238. doi: 10.1002/jmv.1890040308. [DOI] [PubMed] [Google Scholar]
  7. Naot Y., Barnett E. V., Remington J. S. Method for avoiding false-positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. J Clin Microbiol. 1981 Jul;14(1):73–78. doi: 10.1128/jcm.14.1.73-78.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Naot Y., Desmonts G., Remington J. S. IgM enzyme-linked immunosorbent assay test for the diagnosis of congenital Toxoplasma infection. J Pediatr. 1981 Jan;98(1):32–36. doi: 10.1016/s0022-3476(81)80528-8. [DOI] [PubMed] [Google Scholar]
  9. Naot Y., Guptill D. R., Remington J. S. Duration of IgM antibodies to Toxoplasma gondii after acute acquired toxoplasmosis. J Infect Dis. 1982 May;145(5):770–770. doi: 10.1093/infdis/145.2.770. [DOI] [PubMed] [Google Scholar]
  10. Naot Y., Remington J. S. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. J Infect Dis. 1980 Nov;142(5):757–766. doi: 10.1093/infdis/142.5.757. [DOI] [PubMed] [Google Scholar]
  11. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  12. Yolken R. H., Leister F. J. Enzyme immunoassays for measurement of cytomegalovirus immunoglobulin M antibody. J Clin Microbiol. 1981 Oct;14(4):427–432. doi: 10.1128/jcm.14.4.427-432.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van Loon A. M., van der Veen J. Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera. J Clin Pathol. 1980 Jul;33(7):635–639. doi: 10.1136/jcp.33.7.635. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES