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. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983

Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.

R G Wyatt, H D James Jr, A L Pittman, Y Hoshino, H B Greenberg, A R Kalica, J Flores, A Z Kapikian
PMCID: PMC270797  PMID: 6311872

Abstract

Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells or both; 18 were serotyped. Four distinct serotypes were identified by plaque reduction or tube neutralization assay or both, and three of these serotypes were the same as those established previously by plaque reduction, using human rotaviruses cultivated by genetic reassortment with a cultivable bovine rotavirus. Ten human rotavirus strains received from Japan were found to be similar, if not identical, to our candidate prototype strains representing these four human rotavirus serotypes.

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Selected References

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  1. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infect Immun. 1981 Dec;34(3):641–647. doi: 10.1128/iai.34.3.641-647.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beards G. M., Pilfold J. N., Thouless M. E., Flewett T. H. Rotavirus serotypes by serum neutralisation. J Med Virol. 1980;5(3):231–237. doi: 10.1002/jmv.1890050307. [DOI] [PubMed] [Google Scholar]
  3. Beards G. M. Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol. 1982;74(1):65–70. doi: 10.1007/BF01320783. [DOI] [PubMed] [Google Scholar]
  4. Chrystie I. L., Totterdell B., Baker M. J., Scopes J. W., Banatvala J. E. Letter: Rotavirus infections in a maternity unit. Lancet. 1975 Jul 12;2(7924):79–79. doi: 10.1016/s0140-6736(75)90525-5. [DOI] [PubMed] [Google Scholar]
  5. Gaul S. K., Simpson T. F., Woode G. N., Fulton R. W. Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets. J Clin Microbiol. 1982 Sep;16(3):495–503. doi: 10.1128/jcm.16.3.495-503.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  7. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proc Natl Acad Sci U S A. 1981 Jan;78(1):420–424. doi: 10.1073/pnas.78.1.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greenberg H. B., Valdesuso J., van Wyke K., Midthun K., Walsh M., McAuliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol. 1983 Aug;47(2):267–275. doi: 10.1128/jvi.47.2.267-275.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenberg H. B., Wyatt R. G., Kapikian A. Z., Kalica A. R., Flores J., Jones R. Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment. Infect Immun. 1982 Jul;37(1):104–109. doi: 10.1128/iai.37.1.104-109.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983 Jan;39(1):91–99. doi: 10.1128/iai.39.1.91-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hasegawa A., Matsuno S., Inouye S., Kono R., Tsurukubo Y., Mukoyama A., Saito Y. Isolation of human rotaviruses in primary cultures of monkey kidney cells. J Clin Microbiol. 1982 Aug;16(2):387–390. doi: 10.1128/jcm.16.2.387-390.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoshino Y., Wyatt R. G., Scott F. W., Appel M. J. Isolation and characterization of a canine rotavirus. Arch Virol. 1982;72(1-2):113–125. doi: 10.1007/BF01314456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hrdy D. B., Rosen L., Fields B. N. Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice. J Virol. 1979 Jul;31(1):104–111. doi: 10.1128/jvi.31.1.104-111.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kalica A. R., Flores J., Greenberg H. B. Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology. 1983 Feb;125(1):194–205. doi: 10.1016/0042-6822(83)90073-9. [DOI] [PubMed] [Google Scholar]
  15. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  16. Killen H. M., Dimmock N. J. Identification of a neutralization-specific antigen of a calf rotavirus. J Gen Virol. 1982 Oct;62(Pt 2):297–311. doi: 10.1099/0022-1317-62-2-297. [DOI] [PubMed] [Google Scholar]
  17. Kutsuzawa T., Konno T., Suzuki H., Kapikian A. Z., Ebina T., Ishida N. Isolation of human rotavirus subgroups 1 and 2 in cell culture. J Clin Microbiol. 1982 Oct;16(4):727–730. doi: 10.1128/jcm.16.4.727-730.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Matsuno S., Inouye S., Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. doi: 10.1128/jcm.5.1.1-4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rodger S. M., Bishop R. F., Birch C., McLean B., Holmes I. H. Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol. 1981 Feb;13(2):272–278. doi: 10.1128/jcm.13.2.272-278.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sato K., Inaba Y., Miura Y., Tokuhisa S., Matumoto M. Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence. Arch Virol. 1982;73(1):45–50. doi: 10.1007/BF01341726. [DOI] [PubMed] [Google Scholar]
  21. Sato K., Inaba Y., Shinozaki T., Fujii R., Matumoto M. Isolation of human rotavirus in cell cultures: brief report. Arch Virol. 1981;69(2):155–160. doi: 10.1007/BF01315159. [DOI] [PubMed] [Google Scholar]
  22. Thouless M. E., Beards G. M., Flewett T. H. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol. 1982;73(3-4):219–230. doi: 10.1007/BF01318076. [DOI] [PubMed] [Google Scholar]
  23. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  24. Urasawa S., Urasawa T., Taniguchi K. Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infect Immun. 1982 Nov;38(2):781–784. doi: 10.1128/iai.38.2.781-784.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Urasawa T., Urasawa S., Taniguchi K. Sequential passages of human rotavirus in MA-104 cells. Microbiol Immunol. 1981;25(10):1025–1035. doi: 10.1111/j.1348-0421.1981.tb00109.x. [DOI] [PubMed] [Google Scholar]
  26. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. J., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. Human rotavirus type 2: cultivation in vitro. Science. 1980 Jan 11;207(4427):189–191. doi: 10.1126/science.6243190. [DOI] [PubMed] [Google Scholar]
  28. Wyatt R. G., Mebus C. A., Yolken R. H., Kalica A. R., James H. D., Jr, Kapikian A. Z., Chanock R. M. Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus. Science. 1979 Feb 9;203(4380):548–550. doi: 10.1126/science.216077. [DOI] [PubMed] [Google Scholar]

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