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. 1983 Aug;18(2):445–448. doi: 10.1128/jcm.18.2.445-448.1983

Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.

N J Schmidt, J Dennis, V Devlin, D Gallo, J Mills
PMCID: PMC270826  PMID: 6311876

Abstract

Direct immunofluorescence and direct immunoperoxidase staining were equally sensitive and specific for detection of herpes simplex virus antigen in lesion specimens, and each method showed 82% agreement with virus isolation results.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin D. R. Use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection. J Clin Microbiol. 1977 Dec;6(6):571–573. doi: 10.1128/jcm.6.6.571-573.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown S. T., Jaffe H. W., Zaidi A., Filker R., Herrmann K. L., Lylerla H. C., Jove D. F., Budell J. W. Sensitivity and specificity of diagnostic tests for genital infection with herpesvirus hominis. Sex Transm Dis. 1979 Jan-Mar;6(1):10–13. doi: 10.1097/00007435-197901000-00003. [DOI] [PubMed] [Google Scholar]
  3. Elias J. M. A rapid, sensitive myeloperoxidase stain using 4-chloro-1-naphthol. Am J Clin Pathol. 1980 Jun;73(6):797–799. doi: 10.1093/ajcp/73.6.797. [DOI] [PubMed] [Google Scholar]
  4. Hanker J. S., Yates P. E., Metz C. B., Rustioni A. A new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. Histochem J. 1977 Nov;9(6):789–792. doi: 10.1007/BF01003075. [DOI] [PubMed] [Google Scholar]
  5. Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pearson N. S., Fleagle G., Docherty J. J. Detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the Papanicolaou stain. J Clin Microbiol. 1979 Nov;10(5):737–746. doi: 10.1128/jcm.10.5.737-746.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Robinson G., Dawson I. Immunochemical studies of the endocrine cells of the gastrointestinal tract. I. The use and value of peroxidase-conjugated antibody techniques for the localization of gastrin-containing cells in the human pyloric antrum. Histochem J. 1975 Jul;7(4):321–333. doi: 10.1007/BF01007017. [DOI] [PubMed] [Google Scholar]
  8. Rubin S. J., Wende R. D., Rawls W. E. Direct immunofluorescence test for the diagnosis of genital herpesvirus infections. Appl Microbiol. 1973 Sep;26(3):373–375. doi: 10.1128/am.26.3.373-375.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schmidt N. J., Lennette E. H., Woodie J. D., Ho H. H. Immunofluorescent staining in the laboratory diagnosis of varicella-zoster virus infections. J Lab Clin Med. 1965 Sep;66(3):403–412. [PubMed] [Google Scholar]
  10. Thomason B. M., Cowart G. S. Evaluation of polyvinyl alcohols as semipermanent mountants for fluorescent-antibody studies. J Bacteriol. 1967 Feb;93(2):768–769. doi: 10.1128/jb.93.2.768-769.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vestergaard B. F., Thybo H. Rapid diagnosis and typind of herpesvirus hominis by the fluorescent antibody method. Scand J Infect Dis. 1974;6(2):113–116. doi: 10.3109/inf.1974.6.issue-2.03. [DOI] [PubMed] [Google Scholar]

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