Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Sep;18(3):480–485. doi: 10.1128/jcm.18.3.480-485.1983

Monoclonal antibodies against group- and type-specific lipopolysaccharide antigens of Vibrio cholerae O:1.

B Gustafsson, T Holme
PMCID: PMC270838  PMID: 6630437

Abstract

Hybrid cell lines producing monoclonal antibodies against the O-antigenic determinants of Vibrio cholerae O:1 have been established. The specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay inhibition experiments by using lipopolysaccharides from V. cholerae O:1 strains and type strains of groups O:2 and O:21. The anti-A antibody was of the immunoglobulin M (IgM) class, whereas the anti-B and -C antibodies were IgG3. The antibodies had a good agglutinating capacity when tested against V. cholerae O:1 strains in the slide agglutination test.

Full text

PDF
483

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  2. Feeley J. C. Somatic O antigen relationship of Brucella and Vibrio cholerae. J Bacteriol. 1969 Sep;99(3):645–649. doi: 10.1128/jb.99.3.645-649.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gustafsson B., Rosén A., Holme T. Monoclonal antibodies against Vibrio cholerae lipopolysaccharide. Infect Immun. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hisatsune K., Kondo S. Lipopolysaccharides of R mutants isolated from Vibria cholerae. Biochem J. 1980 Jan 1;185(1):77–81. doi: 10.1042/bj1850077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kenne L., Lindberg B., Unger P., Gustafsson B., Holme T. Structural studies of the Vibrio cholerae O-antigen. Carbohydr Res. 1982 Mar 1;100:341–349. doi: 10.1016/s0008-6215(00)81047-2. [DOI] [PubMed] [Google Scholar]
  6. Kenne L., Lindberg B., Unger P., Holme T., Holmgren J. Structural studies of the Vibrio cholerae O-antigen. Carbohydr Res. 1979 Jan;68(1):C14–C16. doi: 10.1016/s0008-6215(00)84073-2. [DOI] [PubMed] [Google Scholar]
  7. Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
  8. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Majumdar A. S., Dutta P., Dutta D., Ghose A. C. Antibacterial and antitoxin responses in the serum and milk of cholera patients. Infect Immun. 1981 Apr;32(1):1–8. doi: 10.1128/iai.32.1.1-8.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Majumdar A. S., Ghose A. C. Protective properties of anticholera antibodies in human colostrum. Infect Immun. 1982 Jun;36(3):962–965. doi: 10.1128/iai.36.3.962-965.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979 Feb;93(1):111–126. doi: 10.1016/0042-6822(79)90280-0. [DOI] [PubMed] [Google Scholar]
  13. Potter M., Pumphrey J. G., Walters J. L. Growth of primary plasmacytomas in the mineral oil-conditioned peritoneal environment. J Natl Cancer Inst. 1972 Jul;49(1):305–308. [PubMed] [Google Scholar]
  14. Redmond J. W. The 4-amino sugars present in the lipopolysaccharides of vibro cholerae and related vibrios. Biochim Biophys Acta. 1978 Sep 6;542(3):378–384. doi: 10.1016/0304-4165(78)90369-0. [DOI] [PubMed] [Google Scholar]
  15. Sakazaki R., Tamura K., Gomez C. Z., Sen R. Serological studies on the cholera group of vibrios. Jpn J Med Sci Biol. 1970 Feb;23(1):13–20. doi: 10.7883/yoken1952.23.13. [DOI] [PubMed] [Google Scholar]
  16. Shimada T., Sakazaki R. R antigen of vibrio cholerae. Jpn J Med Sci Biol. 1973 Aug;26(4):155–160. doi: 10.7883/yoken1952.26.155. [DOI] [PubMed] [Google Scholar]
  17. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  18. Svennerholm A. M., Holmgren J. Synergistic protective effect in rabbits of immunization with Vibrio cholerae lipopolysaccharide and toxin/toxoid. Infect Immun. 1976 Mar;13(3):735–740. doi: 10.1128/iai.13.3.735-740.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]
  20. Winkle S., Refai M., Rohde R. On the antigenic relationship of "Vibrio cholerae" to "Enterobacteriaceae". Ann Inst Pasteur (Paris) 1972 Dec;123(6):775–781. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES