Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Sep;18(3):645–651. doi: 10.1128/jcm.18.3.645-651.1983

Influence of inoculum growth phase on microdilution susceptibility tests.

A L Barry, R E Badal, R W Hawkinson
PMCID: PMC270868  PMID: 6605360

Abstract

Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be convenient and reliable methods for standardizing inocula without preincubation in broth and without turbidity adjustment.

Full text

PDF
648

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker C. N., Thornsberry C., Hawkinson R. W. Inoculum standardization in antimicrobial susceptibility testing: evaluation of overnight agar cultures and the Rapid Inoculum Standardization System. J Clin Microbiol. 1983 Mar;17(3):450–457. doi: 10.1128/jcm.17.3.450-457.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry A. L., Garcia F., Thrupp L. D. An improved single-disk method for testing the antibiotic susceptibility of rapidly-growing pathogens. Am J Clin Pathol. 1970 Feb;53(2):149–158. doi: 10.1093/ajcp/53.2.149. [DOI] [PubMed] [Google Scholar]
  3. Barry A. L., Jones R. N., Gavan T. L. Evaluation of the micro-media system for quantitative antimicrobial drug susceptibility testing: a collaborative study. Antimicrob Agents Chemother. 1978 Jan;13(1):61–69. doi: 10.1128/aac.13.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barry A. L., Joyce L. J., Adams A. P., Benner E. J. Rapid determination of antimicrobial susceptibility for urgent clinical situations. Am J Clin Pathol. 1973 May;59(5):693–699. doi: 10.1093/ajcp/59.5.693. [DOI] [PubMed] [Google Scholar]
  5. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  6. D'Amato R. F., Hochstein L. Evaluation of a rapid inoculum preparation method for agar disk diffusion susceptibility testing. J Clin Microbiol. 1982 Feb;15(2):282–285. doi: 10.1128/jcm.15.2.282-285.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones R. N., Thornsberry C., Barry A. L., Gavan T. L. Evaluation of the Sceptor microdilution antibiotic susceptibility testing system: a collaborative investigation. J Clin Microbiol. 1981 Jan;13(1):184–194. doi: 10.1128/jcm.13.1.184-194.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wicks J. H., Nelson R. L., Krejcarek G. E. Rapid inoculum standardization system: a novel device for standardization of inocula in antimicrobial susceptibility testing. J Clin Microbiol. 1983 Jun;17(6):1114–1119. doi: 10.1128/jcm.17.6.1114-1119.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES