Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Sep;18(3):680–682. doi: 10.1128/jcm.18.3.680-682.1983

Effects of trimethoprim-sulfamethoxazole and incubation atmosphere on isolation of group A streptococci.

C R Libertin, A D Wold, J A Washington 2nd
PMCID: PMC270874  PMID: 6415095

Abstract

The effects of selective media and incubation atmosphere on the isolation of group A beta-hemolytic streptococci were evaluated. A higher percentage of group A streptococci was isolated on sheep blood agar incubated in air than in CO2 or anaerobic atmospheric conditions. Fewer non-group A beta-hemolytic streptococci were isolated on sheep blood agar incubated in air than in CO2 or anaerobically. Group A streptococcal isolation was not significantly affected by different incubation atmospheres on sheep blood agar containing trimethoprim-sulfamethoxazole, but detection time was longer than on sheep blood agar alone. No significant difference was found between isolation of group A streptococci on sheep blood agar incubated in air and that on sheep blood agar containing trimethoprim-sulfamethoxazole and incubated in 5 to 10% CO2; however, more group A streptococci were isolated on sheep blood agar in air within 24 h. Sheep blood agar incubated at 35 degrees C in air is, therefore, recommended for the isolation of group A streptococci from throat swabs.

Full text

PDF
681

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black W. A., Van Buskirk F. Gentamicin as a selective agent for the isolation of beta haemolytic streptococci. J Clin Pathol. 1973 Feb;26(2):154–156. doi: 10.1136/jcp.26.2.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dykstra M. A., McLaughlin J. C., Bartlett R. C. Comparison of media and techniques for detection of group A streptococci in throat swab specimens. J Clin Microbiol. 1979 Feb;9(2):236–238. doi: 10.1128/jcm.9.2.236-238.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gunn B. A., Ohashi D. K., Gaydos C. A., Holt E. S. Selective and enhanced recovery of group A and B streptococci from throat cultures with sheep blood agar containing sulfamethoxazole and trimethoprim. J Clin Microbiol. 1977 Jun;5(6):650–655. doi: 10.1128/jcm.5.6.650-655.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kurzynski T. A., Meise C. K. Evaluation of sulfamethoxazole-trimethoprim blood agar plates for recovery of group A streptocci from throat cultures. J Clin Microbiol. 1979 Feb;9(2):189–193. doi: 10.1128/jcm.9.2.189-193.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kurzynski T., Meise C., Daggs R., Helstad A. Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates. J Clin Microbiol. 1979 Jan;9(1):144–146. doi: 10.1128/jcm.9.1.144-146.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MOODY M. D., ELLIS E. C., UPDYKE E. L. Staining bacterial smears with fluorescent antibody. IV. Grouping streptococci with fluorescent antibody. J Bacteriol. 1958 May;75(5):553–560. doi: 10.1128/jb.75.5.553-560.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Margileth A. M., Pedreira F. A. Office diagnosis of streptococcal upper respiratory tract disease. South Med J. 1975 Apr;68(4):489–494. doi: 10.1097/00007611-197504000-00024. [DOI] [PubMed] [Google Scholar]
  8. McGonagle L. A. Evaluation of a screening procedure for the isolation of beta-hemolytic streptococci. Health Lab Sci. 1974 Apr;11(2):61–64. [PubMed] [Google Scholar]
  9. Murray P. R., Wold A. D., Schreck C. A., Washington JA I. I. Effects of selective media and atmosphere of incubation on the isolation of group A streptococci. J Clin Microbiol. 1976 Jul;4(1):54–56. doi: 10.1128/jcm.4.1.54-56.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosenblatt J. E., Stewart P. R. Lack of activity of sulfamethoxazole and trimethoprim against anaerobic bacteria. Antimicrob Agents Chemother. 1974 Jul;6(1):93–97. doi: 10.1128/aac.6.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanders E. Bacterial interference. I. Its occurrence among the respiratory tract flora and characterization of inhibition of group A streptococci by viridans streptococci. J Infect Dis. 1969 Dec;120(6):698–707. doi: 10.1093/infdis/120.6.698. [DOI] [PubMed] [Google Scholar]
  12. Vincent W. F., Gibbons W. E., Gaafar H. A. Selective medium for the isolation of streptococci from clinical specimens. Appl Microbiol. 1971 Nov;22(5):942–943. doi: 10.1128/am.22.5.942-943.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES