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. 1983 Oct;18(4):961–967. doi: 10.1128/jcm.18.4.961-967.1983

Biochemical markers of the penicillin-induced L phase of a group B, type III Streptococcus sp.

A E Flores, P Ferrieri
PMCID: PMC270939  PMID: 6355170

Abstract

The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase, and CAMP factor in addition to the group- and type-specific antigens. Hemolysin production by the L-phase cells was observed on solid and in liquid media containing sheep erythrocytes. Washed whole L-phase cells hydrolyzed hippuric acid. CAMP factor was detected by the characteristic hemolysis produced on blood agar by L-phase cells or filtered culture supernatants. CAMP factor activity was quantitated by an agar well diffusion system and a macrotube assay with partially purified preparations of CAMP factor and staphylococcal beta-hemolysin. Hyperimmune cow serum neutralized the CAMP activity of the L phase and parent bacterial phase to the same degree, suggesting identity of the CAMP factors. Production of hemolysin, hippuricase, and CAMP factor confirmed the bacterial origin of this L phase. Assay for these biological markers could be used to identify L-phase organisms derived from group B streptococci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernheimer A. W., Avigad L. S., Kim K. S. Staphylococcal sphingomyelinase (beta-hemolysin). Ann N Y Acad Sci. 1974 Jul 31;236(0):292–306. doi: 10.1111/j.1749-6632.1974.tb41499.x. [DOI] [PubMed] [Google Scholar]
  2. Bernheimer A. W., Linder R., Avigad L. S. Nature and mechanism of action of the CAMP protein of group B streptococci. Infect Immun. 1979 Mar;23(3):838–844. doi: 10.1128/iai.23.3.838-844.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown J., Farnsworth R., Wannamaker L. W., Johnson D. W. CAMP factor of group B streptococci: production, assay, and neutralization by sera from immunized rabbits and experimentally infected cows. Infect Immun. 1974 Feb;9(2):377–383. doi: 10.1128/iai.9.2.377-383.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cook J., Fincham W. J. The effect of streptococcal L-form cultures on lymphocytes. Life Sci. 1969 Apr 15;8(8):357–361. doi: 10.1016/0024-3205(69)90059-9. [DOI] [PubMed] [Google Scholar]
  5. FREIMER E. H., KRAUSE R. M., McCARTY M. Studies of L forms and protoplasts of group A streptococci. I. Isolation, growth, and bacteriologic characteristics. J Exp Med. 1959 Dec 1;110:853–874. doi: 10.1084/jem.110.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferrieri P., Gray E. D., Wannamaker L. W. Biochemical and immunological characterization of the extracellular nucleases of group B streptococci. J Exp Med. 1980 Jan 1;151(1):56–68. doi: 10.1084/jem.151.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferrieri P., Wannamaker L. W., Nelson J. Localization and characterization of the hippuricase activity of group B streptococci. Infect Immun. 1973 May;7(5):747–752. doi: 10.1128/iai.7.5.747-752.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HIJMANS W. Absence of the group-specific and the cell-wall polysaccharide antigen in L-phase variants of group D streptococci. J Gen Microbiol. 1962 Apr;28:177–179. doi: 10.1099/00221287-28-1-177. [DOI] [PubMed] [Google Scholar]
  9. Hryniewicz W., Tagg J. R. Bacteriocin production by group a streptococcal L-forms. Antimicrob Agents Chemother. 1976 Dec;10(6):912–914. doi: 10.1128/aac.10.6.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. King J. R., Gooder H. Induction of enterococcal L-forms by the action of lysozyme. J Bacteriol. 1970 Sep;103(3):686–691. doi: 10.1128/jb.103.3.686-691.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Landman O. E., Spiegelman S. ENZYME FORMATION IN PROTOPLASTS OF BACILLUS MEGATERIUM. Proc Natl Acad Sci U S A. 1955 Oct 15;41(10):698–704. doi: 10.1073/pnas.41.10.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marchlewicz B. A., Duncan J. L. Lysis of erythrocytes by a hemolysin produced by a group B Streptococcus sp. Infect Immun. 1981 Dec;34(3):787–794. doi: 10.1128/iai.34.3.787-794.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marchlewicz B. A., Duncan J. L. Properties of a hemolysin produced by group B streptococci. Infect Immun. 1980 Dec;30(3):805–813. doi: 10.1128/iai.30.3.805-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nelson J., Ayoub E. M., Wannamaker L. W. Streptococcal anti-desoxyribonuclease B: microtechnique determination. J Lab Clin Med. 1968 May;71(5):867–873. [PubMed] [Google Scholar]
  15. SHARP J. T., HIJMANS W., DIENES L. Examination of the L forms of group A streptococci for the group-specific polysaccharide and M protein. J Exp Med. 1957 Feb 1;105(2):153–159. doi: 10.1084/jem.105.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith J. A., Willis A. T. Some physiological characters of L forms of Staphylococcus aureus. J Pathol Bacteriol. 1967 Oct;94(2):359–365. doi: 10.1002/path.1700940215. [DOI] [PubMed] [Google Scholar]
  17. Wilkinson H. W. CAMP-disk test for presumptive identification of group B streptococci. J Clin Microbiol. 1977 Jul;6(1):42–45. doi: 10.1128/jcm.6.1.42-45.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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