Abstract
Previously published methods to produce Paracoccidioides brasiliensis antigens for serological tests have yielded antigens of inconsistent quality and have involved the use of special semisynthetic media and growth periods of 1 to 3 months to yield suitable reagents. A simple procedure that uses commercially available potato glucose agar and either SABHI broth (Difco Laboratories) or Trypticase soy broth (BBL Microbiology Systems) inoculated with the mycelial form of P. brasiliensis consistently yielded high-titer antigens in 2 weeks or less. This new method permits the almost exclusive production of an antigen identical to the specific E antigen described by Yarzabal (Yarzabal et al., Sabouradia 14:275-280, 1976) and the apparently equivalent specific antigen 1 described by Restrepo and Moncada (A. Restrepo and L. H. Moncada, Appl. Microbiol. 28:138-144, 1974). In the immunodiffusion test, the rapidly produced antigen demonstrated a sensitivity of 90% by detecting antibody in sera from 103 of 114 proven cases of paracoccidioidomycosis. The specificity of this antigen was 100% because none of 139 sera from patients with heterologous mycotic diseases demonstrated diagnostic precipitins against the P. brasiliensis antigen. In the complement fixation tests, the rapidly produced antigen was not as suitable as the one prepared by the method of Restrepo-Moreno and Schneidau (A. Restrepo-Moreno and J. D. Schneidau, Jr., J. Bacteriol. 93:1741-1748, 1967).
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- Busey J. F., Hinton P. F. Precipitins in histoplasmosis. Am Rev Respir Dis. 1965 Oct;92(4):637–639. doi: 10.1164/arrd.1965.92.4.637. [DOI] [PubMed] [Google Scholar]
- Restrepo-Moreno A., Schneidau J. D., Jr Nature of the skin-reactive principle in culture filtrates prepared from Paracoccidioides brasiliensis. J Bacteriol. 1967 Jun;93(6):1741–1748. doi: 10.1128/jb.93.6.1741-1748.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Restrepo A., Moncada L. H. Characterization of the precipitin bands detected in the immunodiffusion test for paracoccidioidomycosis. Appl Microbiol. 1974 Jul;28(1):138–144. doi: 10.1128/am.28.1.138-144.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yarzabal L. A., Andrieu S., Bout D., Naquira F. Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis. Sabouraudia. 1976 Nov;14(3):275–280. doi: 10.1080/00362177685190411. [DOI] [PubMed] [Google Scholar]