Abstract
An enzyme immunoassay was developed for the detection of human immunoglobulin M (IgM) antibody to different flavivirus antigens. The IgM antibody of human sera was selectively bound to anti-IgM antibody-coated solid-phase plates. Flavivirus IgM antibodies were then detected by use of various enzyme-labeled antigens. The flavivirus antigens (dengue type 2 virus, West Nile virus, and tick-borne encephalitis virus) were produced in suckling mice. The antigens were labeled with horseradish peroxidase by adding the activated enzyme at alkaline pH to sucrose-acetone-treated antigens. Addition of unlabeled mouse brain suspension of uninfected animals to the diluted enzyme-labeled antigens effectively reduced nonspecific binding to the solid phase. In patients with acute flavivirus infections, viral IgM antibody could be demonstrated with high sensitivity. Furthermore, the enzyme-labeled antigen-IgM test showed greater specificity than the hemagglutination inhibition test.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burke D. S., Nisalak A. Detection of Japanese encephalitis virus immunoglobulin M antibodies in serum by antibody capture radioimmunoassay. J Clin Microbiol. 1982 Mar;15(3):353–361. doi: 10.1128/jcm.15.3.353-361.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Dittmar D., Cleary T. J., Castro A. Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies. J Clin Microbiol. 1979 Apr;9(4):498–502. doi: 10.1128/jcm.9.4.498-502.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duermeyer W., van der Veen J. Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet. 1978 Sep 23;2(8091):684–685. doi: 10.1016/s0140-6736(78)92802-7. [DOI] [PubMed] [Google Scholar]
- Edelman R., Pariyanonda A. Human immunoglobulin M antibody in the sero-diagnosis of Japanese encephalitis virus infections. Am J Epidemiol. 1973 Jul;98(1):29–38. doi: 10.1093/oxfordjournals.aje.a121529. [DOI] [PubMed] [Google Scholar]
- Heinz F. X., Roggendorf M., Hofmann H., Kunz C., Deinhardt F. Comparison of two different enzyme immunoassays for detection of immunoglobulin M antibodies against tick-borne encephalitis virus in serum and cerebrospinal fluid. J Clin Microbiol. 1981 Aug;14(2):141–146. doi: 10.1128/jcm.14.2.141-146.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hofmann H., Frisch-Niggemeyer W., Heinz F. Rapid diagnosis of tick-borne encephalitis by means of enzyme linked immunosorbent assay. J Gen Virol. 1979 Mar;42(3):505–511. doi: 10.1099/0022-1317-42-3-505. [DOI] [PubMed] [Google Scholar]
- Krech U., Wilhelm J. A. A solid-phase immunosorbent technique for the rapid detection of rubella IgM by haemagglutination inhibition. J Gen Virol. 1979 Aug;44(2):281–286. doi: 10.1099/0022-1317-44-2-281. [DOI] [PubMed] [Google Scholar]
- Kunz C., Hofmann H. Due Frühdiagnose der Früsommer-meningoenzephalitis (FSME) im Hämaggflutinationshemmungstest durch Behandlung des Serums mit 2-Mercaptoäthanol. Zentralbl Bakteriol Orig A. 1971 Nov;218(3):273–279. [PubMed] [Google Scholar]
- Schmitz H. Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen. J Clin Microbiol. 1982 Aug;16(2):361–366. doi: 10.1128/jcm.16.2.361-366.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmitz H. Improved detection of virus-specific IgM antibodies. Elimination of non-specific IgM binding. J Gen Virol. 1978 Aug;40(2):459–463. doi: 10.1099/0022-1317-40-2-459. [DOI] [PubMed] [Google Scholar]
- Schmitz H., Krainick-Rechert C. M. Simple detection of fluorescent stained IgM in sucrose gradients: demonstration of virus-specific IgM. Intervirology. 1974;3(5-6):353–358. doi: 10.1159/000149772. [DOI] [PubMed] [Google Scholar]
- Schmitz H., von Deimling U., Flehmig B. Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J Gen Virol. 1980 Sep;50(1):59–68. doi: 10.1099/0022-1317-50-1-59. [DOI] [PubMed] [Google Scholar]