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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Aug;20(2):181–184. doi: 10.1128/jcm.20.2.181-184.1984

Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease.

L A Magnarelli, J M Meegan, J F Anderson, W A Chappell
PMCID: PMC271281  PMID: 6386843

Abstract

An enzyme-linked immunosorbent assay was compared with an indirect fluorescent antibody test for its ability to detect antibodies to the Lyme disease spirochete in sera of naturally infected humans, dogs, and white-footed mice and experimentally infected Swiss mice. Ninety-five percent of the total 123 sera analyzed reacted similarly in both tests. For 36 human sera, the correlation coefficient (r = 0.47) for logarithmic transformations of indirect fluorescent antibody and enzyme-linked immunosorbent assay titers was significant at P less than 0.01. Within each mammalian species, mean titers for indirect fluorescent antibody and enzyme-linked immunosorbent assay antibodies were within three-fold. Comparisons of different naturally infected mammals revealed relatively higher average titration endpoints in both tests for patients with Lyme disease. Human sera also had the widest range of titers. Both methods proved satisfactory for serological confirmation of prior spirochetal infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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