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. 1984 Sep;20(3):362–364. doi: 10.1128/jcm.20.3.362-364.1984

Molecular epidemiology of Legionella pneumophila serogroup 1.

R J van Ketel, J ter Schegget, H C Zanen
PMCID: PMC271330  PMID: 6092423

Abstract

The DNA of patient and environmental isolates of Legionella pneumophila serogroup 1 was analyzed by restriction endonuclease cleavage. The electrophoretic patterns of the DNA digests of isolates from a group of patients with Legionnaires disease acquired in a hospital were indistinguishable from one another and were identical to the DNA pattern of a strain isolated from the hot water supply of the hospital. On the other hand, they were easily differentiated from strains isolated from patients and hot water supplies in other hospitals in the same city. The homogeneity of populations of L. pneumophila serogroup 1 colonizing plumbing systems was also investigated by DNA restriction endonuclease analysis in three hospitals. We distinguished two subtypes in one hospital; the two other hospitals had homogeneous populations. Restriction endonuclease digest analysis of L. pneumophila serogroup 1 DNA enables subtyping and appears to be a useful method for examining the epidemiology of outbreaks of Legionnaires disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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