Abstract
Enzyme-linked immunosorbent assays (ELISAs) with antibodies specific to either cholera enterotoxin (CT) of Vibrio cholerae or heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli were developed to detect LT and CT, respectively. With these ELISA systems, LT and CT could be detected only with the respective specific antibody. Both antibody ELISA and ganglioside ELISA were used for differential detection of LT and CT, but the former method seemed to be more specific. By this ELISA, as little as 0.1 ng of purified LT or CT could be detected per ml. The type of toxins in fluids in intestinal loops of experimental animals challenged with living cells of either V. cholerae or LT-producing E. coli was identified correctly by this ELISA. These results suggest that the ELISA systems reported here could be used to detect and differentiate CT and LT in unknown samples; it could also be used for assaying toxins in stool specimens for the diagnosis of diarrhea due to V. cholerae or LT-producing E. coli directly, without or before bacterial isolation.
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Selected References
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- Craig J. P. A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera. Nature. 1965 Aug 7;207(997):614–616. doi: 10.1038/207614a0. [DOI] [PubMed] [Google Scholar]
- Gorbach S. L., Banwell J. G., Chatterjee B. D., Jacobs B., Sack R. B. Acute undifferentiated human diarrhea in the tropics. I. Alterations in intestinal micrflora. J Clin Invest. 1971 Apr;50(4):881–889. doi: 10.1172/JCI106560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Akhtar Q., Glass R. I., Kibriya A. K. A simple assay to detect Escherichia coli producing heat labile enterotoxin: results of a field study of the Biken tests in Bangladesh. Lancet. 1981 Sep 19;2(8247):609–610. doi: 10.1016/s0140-6736(81)92745-8. [DOI] [PubMed] [Google Scholar]
- Honda T., Arita M., Takeda Y., Miwatani T. Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin. J Clin Microbiol. 1982 Jul;16(1):60–62. doi: 10.1128/jcm.16.1.60-62.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Takeda Y., Miwatani T. Isolation of special antibodies which react only with homologous enterotoxins from Vibrio cholerae and Enterotoxigenic Escherichia coli. Infect Immun. 1981 Nov;34(2):333–336. doi: 10.1128/iai.34.2.333-336.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merson M. H., Yolken R. H., Sack R. B., Froehlich J. L., Greenberg H. B., Huq I., Black R. W. Detection of Escherichia coli enterotoxins in stools. Infect Immun. 1980 Jul;29(1):108–113. doi: 10.1128/iai.29.1.108-113.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sack D. A., Huda S., Neogi P. K., Daniel R. R., Spira W. M. Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin. J Clin Microbiol. 1980 Jan;11(1):35–40. doi: 10.1128/jcm.11.1.35-40.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sack R. B. Enterotoxigenic Escherichia coli: identification and characterization. J Infect Dis. 1980 Aug;142(2):279–286. doi: 10.1093/infdis/142.2.279. [DOI] [PubMed] [Google Scholar]
- Stoll B. J., Holmgren J., Bardhan P. K., Huq I., Greenough W. B., 3rd, Fredman P., Svennerholm L. Binding of intraluminal toxin in cholera: trial of GM1 ganglioside charcoal. Lancet. 1980 Oct 25;2(8200):888–891. doi: 10.1016/s0140-6736(80)92049-8. [DOI] [PubMed] [Google Scholar]
- Svennerholm A. M., Wiklund G. Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1983 Apr;17(4):596–600. doi: 10.1128/jcm.17.4.596-600.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takeda Y., Honda T., Taga S., Miwatani T. In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin. Infect Immun. 1981 Nov;34(2):341–346. doi: 10.1128/iai.34.2.341-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]