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. 1984 Nov;20(5):862–865. doi: 10.1128/jcm.20.5.862-865.1984

Comparison of media for isolation of Ureaplasma urealyticum and genital Mycoplasma species.

V Fiacco, M J Miller, E Carney, W J Martin
PMCID: PMC271460  PMID: 6511871

Abstract

A total of 484 frozen patient specimens originally positive for Ureaplasma urealyticum or Mycoplasma spp. or both were recultured, and the results were compared on the following media: Shepard's A7 agar, modified phenol red-urea, SP-4-urea, modified phenol red-arginine, and SP-4-arginine broths. Of 351 specimens positive for U. urealyticum, 30 (8.5%) were detected only in one or more of the broth media, whereas 117 (33%) were positive only on A7 agar. Separate use of the SP-4-urea broth or modified phenol red-urea broth isolated all but 1 and 2, respectively, of the negative A7 agar cultures. Of the 76 specimens positive for large colony Mycoplasma spp., 58 (76.3%) were not grown on the primary plating (A7) agar. Of 73 broth isolates, 73 (100%) grew in SP-4-arginine broth, and 64 (87.7%) grew in modified phenol red-arginine broth. Modified SP-4 broth appears to be a useful supplement to the A7 plating medium commonly used in the diagnostic laboratory for the isolation of U. urealyticum and Mycoplasma spp.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black F. T., Rasmussen O. G. Occurrence of T-strains and other mycoplasmata in non-gonococcal urethritis. Br J Vener Dis. 1968 Dec;44(4):324–330. doi: 10.1136/sti.44.4.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun P., Klein J. O., Lee Y. H., Kass E. H. Methodologic investigations and prevalence of genital mycoplasmas in pregnancy. J Infect Dis. 1970 Apr;121(4):391–400. doi: 10.1093/infdis/121.4.391. [DOI] [PubMed] [Google Scholar]
  3. Kundsin R. B., Parreno A., Poulin S. Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978 Oct;8(4):445–453. doi: 10.1128/jcm.8.4.445-453.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Leland D. S., Lapworth M. A., Jones R. B., French M. L. Comparative evaluation of media for isolation of ureaplasma urealyticum and genital mycoplasma species. J Clin Microbiol. 1982 Oct;16(4):709–714. doi: 10.1128/jcm.16.4.709-714.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MADOFF S. Isolation and identification of PPLO. Ann N Y Acad Sci. 1960 Jan 15;79:383–392. doi: 10.1111/j.1749-6632.1960.tb42702.x. [DOI] [PubMed] [Google Scholar]
  6. Møller B. R. The role of mycoplasmas in the upper genital tract of women. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):281–284. [PubMed] [Google Scholar]
  7. Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rottem S., Pfendt E. A., Hayflick L. Sterol requirements of T-strain mycoplasmas. J Bacteriol. 1971 Jan;105(1):323–330. doi: 10.1128/jb.105.1.323-330.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shepard M. C., Lunceford C. D. Urease color test medium U-9 for the detection and identification of "T" mycoplasms in clinical material. Appl Microbiol. 1970 Oct;20(4):539–543. doi: 10.1128/am.20.4.539-543.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taylor-Robinson D., Addey J. P., Goodwin C. S. Comparison of technics for the isolation of T-strain mycoplasmas. Nature. 1969 Apr 19;222(5190):274–275. doi: 10.1038/222274a0. [DOI] [PubMed] [Google Scholar]
  12. Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (second of two parts). N Engl J Med. 1980 May 8;302(19):1063–1067. doi: 10.1056/NEJM198005083021905. [DOI] [PubMed] [Google Scholar]
  13. Tully J. G., Rose D. L., Whitcomb R. F., Wenzel R. P. Enhanced isolation of Mycoplasma pneumoniae from throat washings with a newly-modified culture medium. J Infect Dis. 1979 Apr;139(4):478–482. doi: 10.1093/infdis/139.4.478. [DOI] [PubMed] [Google Scholar]
  14. Tully J. G., Taylor-Robinson D., Cole R. M., Rose D. L. A newly discovered mycoplasma in the human urogenital tract. Lancet. 1981 Jun 13;1(8233):1288–1291. doi: 10.1016/s0140-6736(81)92461-2. [DOI] [PubMed] [Google Scholar]
  15. Tully J. G., Taylor-Robinson D., Rose D. L., Furr P. M., Hawkins D. A. Evaluation of culture media for the recovery of Mycoplasma hominis from the human urogenital tract. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):256–260. [PubMed] [Google Scholar]
  16. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L. Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science. 1977 Mar 4;195(4281):892–894. doi: 10.1126/science.841314. [DOI] [PubMed] [Google Scholar]
  17. Yajko D. M., Balston E., Wood D., Sweet R. L., Hadley W. K. Evaluation of PPLO, A7B, E, and NYC agar media for the isolation of Ureaplasma urealyticum and Mycoplasma species from the genital tract. J Clin Microbiol. 1984 Jan;19(1):73–76. doi: 10.1128/jcm.19.1.73-76.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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