Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Mar;21(3):298–301. doi: 10.1128/jcm.21.3.298-301.1985

Detection of Mycoplasma hominis septicemia by radiometric blood culture.

M F Smaron, S Boonlayangoor, C H Zierdt
PMCID: PMC271650  PMID: 3980685

Abstract

The ease with which Mycoplasma hominis can be recovered and the frequency of its occurrence in septicemia may not be fully appreciated. We detected the growth of M. hominis radiometrically with an automated blood culture instrument. The organism grew in both aerobic and anaerobic culture media, but the cultures were not visibly positive. It was necessary to stain the cultures with acridine orange to visualize M. hominis and to subculture them on Columbia base sheep blood agar to confirm the positive growth index indicated by the instrument. Sodium polyanetholesulfonate inhibited the growth of M. hominis and is not recommended for use as the anticoagulant when blood is cultured for Mycoplasma spp.

Full text

PDF
301

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bøe O., Iversen O. E., Mehl A. Septicemia due to Mycoplasma hominis. Scand J Infect Dis. 1983;15(1):87–90. doi: 10.3109/inf.1983.15.issue-1.14. [DOI] [PubMed] [Google Scholar]
  2. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  3. Carski T. R., Broman R. L., Del Giudice R. A. A note on positive BACTEC readings produced by Mycoplasma. J Appl Bacteriol. 1981 Aug;51(1):41–43. doi: 10.1111/j.1365-2672.1981.tb00906.x. [DOI] [PubMed] [Google Scholar]
  4. DeGirolami P. C., Madoff S. Mycoplasma hominis septicemia. J Clin Microbiol. 1982 Sep;16(3):566–567. doi: 10.1128/jcm.16.3.566-567.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friis H., Plesner A., Scheibel J., Justesen T., Lind K. Mycoplasma hominis septicaemia. Br Med J (Clin Res Ed) 1983 Jun 25;286(6383):2013–2014. doi: 10.1136/bmj.286.6383.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mårdh P. A., Weström L. Tubal and cervical cultures in acute salpingitis with special reference to Mycoplasma hominis and T-strain mycoplasmas. Br J Vener Dis. 1970 Jun;46(3):179–186. [PMC free article] [PubMed] [Google Scholar]
  7. Platt R., Lin J. S., Warren J. W., Rosner B., Edelin K. C., McCormack W. M. Infection with Mycoplasma hominis in postpartum fever. Lancet. 1980 Dec 6;2(8206):1217–1221. doi: 10.1016/s0140-6736(80)92479-4. [DOI] [PubMed] [Google Scholar]
  8. Simberkoff M. S., Toharsky B. Mycoplasmemia in adult male patients. JAMA. 1976 Nov 29;236(22):2522–2524. [PubMed] [Google Scholar]
  9. Thomsen A. C. Occurrence of mycoplasmas in urinary tracts of patients with acute pyelonephritis. J Clin Microbiol. 1978 Jul;8(1):84–88. doi: 10.1128/jcm.8.1.84-88.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ti T. Y., Dan M., Stemke G. W., Robertson J., Goldsand G. Isolation of Mycoplasma hominis from the blood of men with multiple trauma and fever. JAMA. 1982 Jan 1;247(1):60–61. [PubMed] [Google Scholar]
  11. Tully J. G., Brown M. S., Sheagren J. N., Young V. M., Wolff S. M. Septicemia due to Mycoplasma hominis type 1. N Engl J Med. 1965 Sep 16;273(12):648–650. doi: 10.1056/NEJM196509162731207. [DOI] [PubMed] [Google Scholar]
  12. Wallace R. J., Jr, Alpert S., Browne K., Lin J. S., McCormack W. M. Isolation of Mycoplasma hominis from blood cultures in patients with postpartum fever. Obstet Gynecol. 1978 Feb;51(2):181–185. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES