Abstract
The value of incubating blood culture broths for more than 7 days was analyzed. A total of 20,155 blood cultures, consisting of a vented Roche tryptic soy broth (R-TSB) bottle and an unvented Difco thiol broth bottle, was processed; 2,509 organisms were recovered in the R-TSB bottles, and 1,865 organisms in the thiol bottles. Only 32 organisms isolated in the R-TSB bottles and 10 organisms isolated in the thiol bottles were detected after incubation for more than 7 days; 15 of the 32 isolates in the R-TSB bottles and all 10 of the isolates in the thiol bottles were either recovered in other blood cultures or were not considered to be clinically significant. Thus, incubation of the R-TSB bottles and unvented thiol bottles for more than 7 days is unnecessary for the detection of most clinically significant septicemias.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bille J., Stockman L., Roberts G. D., Horstmeier C. D., Ilstrup D. M. Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. J Clin Microbiol. 1983 Sep;18(3):469–471. doi: 10.1128/jcm.18.3.469-471.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geraci J. E., Wilson W. R., Washington J. A., 2nd Infective endocarditis caused by Actinobacillus actinomycetemcomitans: report of four cases. Mayo Clin Proc. 1980 Jul;55(7):415–419. [PubMed] [Google Scholar]
- Gross P. A. Detection of bacteremia: technical aspects of the blood culture. Infect Control. 1982 Jul-Aug;3(4):284–286. doi: 10.1017/s0195941700056319. [DOI] [PubMed] [Google Scholar]
- Hall M., Warren E., Washington J. A., 2nd Detection of bacteremia with liquid media containing sodium polyanetholsulfonate. Appl Microbiol. 1974 Jan;27(1):187–191. doi: 10.1128/am.27.1.187-191.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henry N. K., Grewell C. M., McLimans C. A., Washington J. A., 2nd Comparison of the Roche Septi-Chek blood culture bottle with a brain heart infusion biphasic medium bottle and with a tryptic soy broth bottle. J Clin Microbiol. 1984 Mar;19(3):315–317. doi: 10.1128/jcm.19.3.315-317.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henry N. K., McLimans C. A., Wright A. J., Thompson R. L., Wilson W. R., Washington J. A., 2nd Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube. J Clin Microbiol. 1983 May;17(5):864–869. doi: 10.1128/jcm.17.5.864-869.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray P. R., Sondag J. E. Evaluation of routine subcultures of macroscopically negative blood cultures for detection of anaerobes. J Clin Microbiol. 1978 Oct;8(4):427–430. doi: 10.1128/jcm.8.4.427-430.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaller M. A., Sibley T. K., Westfall L. M., Hoppe-Bauer J. E., Keating M. A., Murray P. R. Clinical laboratory comparison of a slide blood culture system with a conventional broth system. J Clin Microbiol. 1982 Sep;16(3):525–530. doi: 10.1128/jcm.16.3.525-530.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaller M. A., Westfall L. M., Murray P. R. Value of routine aerobic subculturing of unvented blood culture bottles. J Clin Microbiol. 1983 Apr;17(4):601–604. doi: 10.1128/jcm.17.4.601-604.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosner R. Growth patterns of a wide spectrum of organisms encountered in clinical blood cultures using both hypertonic and isotonic media. Am J Clin Pathol. 1976 May;65(5):706–710. doi: 10.1093/ajcp/65.5.706. [DOI] [PubMed] [Google Scholar]
- Smith J. A., Roberts F. J., Ngui-Yen J. Comparison of a radiometric and a broth-slide system for aerobic blood culture. J Clin Microbiol. 1983 Jul;18(1):217–218. doi: 10.1128/jcm.18.1.217-218.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd The role of the microbiology laboratory in the diagnosis and antimicrobial treatment of infective endocarditis. Mayo Clin Proc. 1982 Jan;57(1):22–32. [PubMed] [Google Scholar]