Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 Sep;14(3):241–246. doi: 10.1128/jcm.14.3.241-246.1981

Difference in antibody reactivity between complement fixation and immune adherence hemagglutination tests with virus antigens.

S Inouye, S Matsuno, R Kono
PMCID: PMC271948  PMID: 6270189

Abstract

Complement-fixing (CF) immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) did not show the reactivity of immune adherence hemagglutination (IAHA). Early immunoglobulin G CF antibody produced both in patients and in guinea pigs experimentally infected with Japanese encephalitis virus (a flavivirus) had weak reactivity in IAHA test. However, late antibody showed higher titers by IAHA than by CF. These results suggested that early antibodies with lower affinity are inefficient in the IAHA reaction. The implications of this study are: (i) a low ratio of IAHA/CF antibody titers in a serum suggests a recent rotavirus infection; (ii) the IAHA reaction is more type specific than the CF reaction for identifying the serotype of antigenically cross-reacting viruses with hyperimmune sera.

Full text

PDF
245

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe Y., Inouye S. Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis. J Clin Microbiol. 1979 Feb;9(2):284–287. doi: 10.1128/jcm.9.2.284-287.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BELLANTI J. A., RUSS S. B., HOLMES G. E., BUESCHER E. L. THE NATURE OF ANTIBODIES FOLLOWING EXPERIMENTAL ARBOVIRUS INFECTION IN GUINEA PIGS. J Immunol. 1965 Jan;94:1–11. [PubMed] [Google Scholar]
  3. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  4. Booth J. C., Hannington G., Tryhorn Y., Stern H. Cytomegalovirus complement-fixing IgM antibody. J Med Virol. 1980;5(3):183–193. doi: 10.1002/jmv.1890050303. [DOI] [PubMed] [Google Scholar]
  5. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  6. Capel P. J., Groeneboer O., Grosveld G., Pondman K. W. The binding of activated C3 to polysaccharides and immunoglobulins. J Immunol. 1978 Dec;121(6):2566–2572. [PubMed] [Google Scholar]
  7. Coursaget P., Drucker J., Maupas P., Hibon P., Goudeau A., Bernard D., Sauvage D., Lelord G. Epidemiological and serological study of hepatitis A virus outbreaks in France: a comparison between immunoadherence and radioimmunoassay. J Hyg (Lond) 1981 Apr;86(2):155–162. doi: 10.1017/s0022172400068868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cremer N. E., Hoffman M., Lennette E. H. Analysis of antibody assay methods and classes of viral antibodies in serodiagnosis of cytomegalovirus infection. J Clin Microbiol. 1978 Aug;8(2):153–159. doi: 10.1128/jcm.8.2.153-159.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Czop J., Nussenzweig V. Studies on the mechanism of solubilization of immune precipitates by serum. J Exp Med. 1976 Mar 1;143(3):615–630. doi: 10.1084/jem.143.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dienstag J. L., Alling D. W., Purcell R. H. Quantitation of antibody to hepatitis A antigen by immune electron microscopy. Infect Immun. 1976 Apr;13(4):1209–1213. doi: 10.1128/iai.13.4.1209-1213.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Drucker J., Smallwood L., Lorenz D., Tabor E., Coursaget P., Maupas P., Barker L. F., Gerety R. J. Serologic response in human hepatitis A: detection of antibody by radioimmunoassay and immune adherence hemagglutination. J Med Virol. 1979;4(1):51–58. doi: 10.1002/jmv.1890040107. [DOI] [PubMed] [Google Scholar]
  12. Gerna G., Cereda P. M., Cattaneo E., Achilli G., Gerna M. T. Antibody to early antigens of varicella-zoster virus during varicella and zoster. J Infect Dis. 1979 Jul;140(1):33–41. doi: 10.1093/infdis/140.1.33. [DOI] [PubMed] [Google Scholar]
  13. Gershon A. A., Kalter Z. G., Steinberg S., Kuhns W. J. Detection of antibody to Varicella-Zoster virus by immune adherence hemagglutination. Proc Soc Exp Biol Med. 1976 Apr;151(4):762–765. doi: 10.3181/00379727-151-39302. [DOI] [PubMed] [Google Scholar]
  14. Hansson B. G., Calhoun J. K., Wong D. C., Feinstone S. M., Purcell R. H., Pannuti C. S., Pereira J. L., Koff R. S., Dienstag J. L., Iwarson S. Serodiagnosis of viral hepatitis A by a solid-phase radioimmunoassay specific for IgM antibodies. Scand J Infect Dis. 1981;13(1):5–9. doi: 10.1080/00365548.1981.11690359. [DOI] [PubMed] [Google Scholar]
  15. Hierholzer J. C., Suggs M. T. Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. Appl Microbiol. 1969 Nov;18(5):816–823. doi: 10.1128/am.18.5.816-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Inouye S., Matsuno S., Hasegawa A., Miyamura K., Kono R., Rosen L. Serotyping of dengue viruses by an immune adherence hemagglutination test. Am J Trop Med Hyg. 1980 Nov;29(6):1389–1393. doi: 10.4269/ajtmh.1980.29.1389. [DOI] [PubMed] [Google Scholar]
  17. Ito M., Tagaya I. Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies. Jpn J Med Sci Biol. 1966 Jun;19(3):109–126. doi: 10.7883/yoken1952.19.109. [DOI] [PubMed] [Google Scholar]
  18. Konno T., Imai A., Suzuki H., Ishida N. Letter: Mercaptoethanol-sensitive antibody to reovirus-like agents in acute epidemic gastroenteritis. Lancet. 1975 Dec 27;2(7948):1312–1312. doi: 10.1016/s0140-6736(75)90654-6. [DOI] [PubMed] [Google Scholar]
  19. Krugman S., Friedman H., Lattimer C. Viral hepatitis, type A. Identification by specific complement fixation and immune adherence tests. N Engl J Med. 1975 May 29;292(22):1141–1143. doi: 10.1056/NEJM197505292922201. [DOI] [PubMed] [Google Scholar]
  20. Lennette E. T., Lennette D. A. Immune adherence hemagglutination: alternative to complement-fixation serology. J Clin Microbiol. 1978 Mar;7(3):282–285. doi: 10.1128/jcm.7.3.282-285.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mao J. S., Yu P. H., Ding Z. S., Chen N. L., Huang B. Z., Xie R. Y., Chai S. A. Patterns of shedding of hepatitis A virus antigen in feces and of antibody responses in patients with naturally acquired type A hepatitis. J Infect Dis. 1980 Nov;142(5):654–659. doi: 10.1093/infdis/142.5.654. [DOI] [PubMed] [Google Scholar]
  22. Matsuno S., Nagayoshi S. Quantitative estimation of infantile gastroenteritis virus antigens in stools by immune adherence hemagglutination test. J Clin Microbiol. 1978 Mar;7(3):310–311. doi: 10.1128/jcm.7.3.310-311.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mayumi M., Okochi K., Nishioka K. Detection of Australia antigen by means of immune adherence haemagglutination test. Vox Sang. 1971 Feb;20(2):178–181. doi: 10.1111/j.1423-0410.1971.tb00549.x. [DOI] [PubMed] [Google Scholar]
  24. Miller G. W., Nussenzweig V. A new complement function: solubilization of antigen-antibody aggregates. Proc Natl Acad Sci U S A. 1975 Feb;72(2):418–422. doi: 10.1073/pnas.72.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Miller W. J., Provost P. J., McAleer W. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations. Proc Soc Exp Biol Med. 1975 May;149(1):254–261. doi: 10.3181/00379727-149-38783. [DOI] [PubMed] [Google Scholar]
  26. Moritsugu Y., Dienstag J. L., Valdesuso J., Wong D. C., Wagner J., Routenberg J. A., Purcell R. H. Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination. Infect Immun. 1976 Mar;13(3):898–908. doi: 10.1128/iai.13.3.898-908.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rakela J., Redeker A. G., Edwards V. M., Decker R., Overby L. R., Mosley J. W. Hepatits A virus infection in fulminant hepatitis and chronic active hepatitis. Gastroenterology. 1978 May;74(5 Pt 1):879–882. [PubMed] [Google Scholar]
  28. Rakela J., Stevenson D., Edwards V. M., Gordon I., Mosley J. W. Antibodies to hepatitis A virus: patterns by two procedures. J Clin Microbiol. 1977 Jan;5(1):110–111. doi: 10.1128/jcm.5.1.110-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Suzuki T., Yoshida Y., Oda K., Takamiya A. [Application of immune adherence hemagglutination test for typing of enteroviruses (author's transl)]. Uirusu. 1979 Dec;29(2):153–154. [PubMed] [Google Scholar]
  30. Takahashi M., Tack B. F., Nussenzweig V. Requirements for the solubilization of immune aggregates by complement: assembly of a factor B-dependent C3-convertase on the immune complexes. J Exp Med. 1977 Jan 1;145(1):86–100. doi: 10.1084/jem.145.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES