Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Feb;15(2):249–252. doi: 10.1128/jcm.15.2.249-252.1982

Enzyme-linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens.

L A Joens, N A Nord, J M Kinyon, I T Egan
PMCID: PMC272070  PMID: 7040447

Abstract

The enzyme-linked immunosorbent assay (ELISA) was evaluated and compared with the microtitration agglutination test for the detection of swine antibody to Treponema hyodysenteriae lipopolysaccharide antigens. Cells of T. hyodysenteriae serotypes 1 and 2 were extracted with hot phenol-water (68 degrees C). The lipopolysaccharide fraction from the aqueous phase was coated on plastic wells at concentrations of 1 micrograms (serotype 1) and 10 micrograms (serotype 2) of carbohydrate per ml. The ELISA was serotype specific when lipopolysaccharide antigens were reacted against sera from convalescent swine. Seroconversion of infected pigs was detectable with the ELISA within 1 to 2 weeks postinoculation and with the microtitration agglutination test 2 to 3 weeks postinoculation. Antibody titers could be detected in convalescent pigs as long as 19 weeks postinoculation by the ELISA and 12 to 13 weeks postinoculation by the microtitration agglutination test. Therefore, the ELISA may be useful for the detection of asymptomatic carriers.

Full text

PDF
251

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander T. J., Taylor D. J. The clinical signs, diagnosis and control of swine dysentery. Vet Rec. 1969 Jul 19;85(3):59–63. doi: 10.1136/vr.85.3.59. [DOI] [PubMed] [Google Scholar]
  2. Baum D. H., Joens L. A. Serotypes of beta-hemolytic Treponema hyodysenteriae. Infect Immun. 1979 Sep;25(3):792–796. doi: 10.1128/iai.25.3.792-796.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlsson H. E., Hurvell B., Lindberg A. A. Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):168–176. doi: 10.1111/j.1699-0463.1976.tb00016.x. [DOI] [PubMed] [Google Scholar]
  4. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. J Infect Dis. 1977 Oct;136 (Suppl):S337–S340. doi: 10.1093/infdis/136.supplement_2.s337. [DOI] [PubMed] [Google Scholar]
  6. Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
  7. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  8. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  9. Hunter D., Saunders C. N. Serum agglutination test for swine dysentery. Vet Rec. 1973 Jul 28;93(4):107–107. doi: 10.1136/vr.93.4.107-a. [DOI] [PubMed] [Google Scholar]
  10. Joens L. A., Harris D. L., Baum D. H. Immunity to Swine dysentery in recovered pigs. Am J Vet Res. 1979 Oct;40(10):1352–1354. [PubMed] [Google Scholar]
  11. Joens L. A., Harris D. L., Kinyon J. M., Kaeberle M. L. Microtitration agglutination for detection of Treponema hyodysenteriae antibody. J Clin Microbiol. 1978 Sep;8(3):293–298. doi: 10.1128/jcm.8.3.293-298.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Joens L. A., Songer J. G., Harris D. L., Glock R. D. Experimental infection with Treponema hyodysenteriae in guinea pigs. Infect Immun. 1978 Oct;22(1):132–135. doi: 10.1128/iai.22.1.132-135.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Joens L. A., Songer J. G., Harris D. L., Kinyon J. M. Comparison of selective culture and serologic agglutination of Treponema hyodysenteriae for diagnosis of swine dysentery. Vet Rec. 1979 Nov 17;105(20):463–465. doi: 10.1136/vr.105.20.463. [DOI] [PubMed] [Google Scholar]
  14. Keren D. F. Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens. Infect Immun. 1979 May;24(2):441–448. doi: 10.1128/iai.24.2.441-448.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lamb V. L., Jones L. M., Schurig G. G., Berman D. T. Enzyme-linked immunosorbent assay for bovine immunoglobulin subclass-specific response to Brucella abortus lipopolysaccharides. Infect Immun. 1979 Oct;26(1):240–247. doi: 10.1128/iai.26.1.240-247.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Songer J. G., Harris D. L. Transmission of swine dysentery by carrier pigs. Am J Vet Res. 1978 Jun;39(6):913–916. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES