Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Feb;15(2):347–349. doi: 10.1128/jcm.15.2.347-349.1982

Problems Associated with Counterimmunoelectrophoresis Assays for Detecting Clostridium difficile Toxin

Susan E H West 1, Tracy D Wilkins 1
PMCID: PMC272093  PMID: 7068832

Abstract

The antitoxins currently used for the detection of Clostridium difficile by counterimmunoelectrophoresis react with other C. difficile antigens in addition to the toxins produced by the bacterium.

Full text

PDF
348

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett J. G., Chang T. W., Gurwith M., Gorbach S. L., Onderdonk A. B. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med. 1978 Mar 9;298(10):531–534. doi: 10.1056/NEJM197803092981003. [DOI] [PubMed] [Google Scholar]
  2. Bartlett J. G., Taylor N. S., Chang T., Dzink J. Clinical and laboratory observations in Clostridium difficile colitis. Am J Clin Nutr. 1980 Nov;33(11 Suppl):2521–2526. doi: 10.1093/ajcn/33.11.2521. [DOI] [PubMed] [Google Scholar]
  3. Chang T. W., Bartlett J. G., Gorbach S. L., Onderdonk A. B. Clindamycin-induced enterocolitis in hamsters as a model of pseudomembranous colitis in patients. Infect Immun. 1978 May;20(2):526–529. doi: 10.1128/iai.20.2.526-529.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang T. W., Lauermann M., Bartlett J. G. Cytotoxicity assay in antibiotic-associated colitis. J Infect Dis. 1979 Nov;140(5):765–770. doi: 10.1093/infdis/140.5.765. [DOI] [PubMed] [Google Scholar]
  5. Ehrich M., Van Tassell R. L., Libby J. M., Wilkins T. D. Production of Clostridium difficile antitoxin. Infect Immun. 1980 Jun;28(3):1041–1043. doi: 10.1128/iai.28.3.1041-1043.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. George R. H., Symonds J. M., Dimock F., Brown J. D., Arabi Y., Shinagawa N., Keighley M. R., Alexander-Williams J., Burdon D. W. Identification of Clostridium difficile as a cause of pseudomembranous colitis. Br Med J. 1978 Mar 18;1(6114):695–695. doi: 10.1136/bmj.1.6114.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. George W. L., Sutter V. L., Citron D., Finegold S. M. Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol. 1979 Feb;9(2):214–219. doi: 10.1128/jcm.9.2.214-219.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. George W. L., Sutter V. L., Goldstein E. J., Ludwig S. L., Finegold S. M. Aetiology of antimicrobial-agent-associated colitis. Lancet. 1978 Apr 15;1(8068):802–803. doi: 10.1016/s0140-6736(78)93001-5. [DOI] [PubMed] [Google Scholar]
  9. Larson H. E., Price A. B., Honour P., Borriello S. P. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet. 1978 May 20;1(8073):1063–1066. doi: 10.1016/s0140-6736(78)90912-1. [DOI] [PubMed] [Google Scholar]
  10. Monthony J. F., Wallace E. G., Allen D. M. A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in agarose gels. Clin Chem. 1978 Oct;24(10):1825–1827. [PubMed] [Google Scholar]
  11. Poxton I. R., Byrne M. D. Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species. J Gen Microbiol. 1981 Jan;122(1):41–46. doi: 10.1099/00221287-122-1-41. [DOI] [PubMed] [Google Scholar]
  12. Rifkin G. D., Fekety F. R., Silva J., Jr Antibiotic-induced colitis implication of a toxin neutralised by Clostridium sordellii antitoxin. Lancet. 1977 Nov 26;2(8048):1103–1106. doi: 10.1016/s0140-6736(77)90547-5. [DOI] [PubMed] [Google Scholar]
  13. Ryan R. W., Kwasnik I., Tilton R. C. Rapid detection of Clostridium difficile toxin in human feces. J Clin Microbiol. 1980 Dec;12(6):776–779. doi: 10.1128/jcm.12.6.776-779.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Welch D. F., Menge S. K., Matsen J. M. Identification of toxigenic Clostridium difficile by counterimmunoelectrophoresis. J Clin Microbiol. 1980 May;11(5):470–473. doi: 10.1128/jcm.11.5.470-473.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES