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. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982

Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors.

H Lior, D L Woodward, J A Edgar, L J Laroche, P Gill
PMCID: PMC272187  PMID: 7096555

Abstract

A serotyping scheme for Campylobacter jejuni was developed based on slide agglutination of live bacteria with whole cell antisera absorbed with homologous heated and heterologous unheated cross-reactive antigens. Among 815 isolates from human and nonhuman sources, 21 serogroups were recognized. Of the 615 isolates from human cases of gastroenteritis, 529 (86%) were typable; 455 strains agglutinated in 20 single antisera, whereas 74 isolates agglutinated in various pairs of antisera, allowing subdivision of some main serogroups into subserogroups. Of the 200 isolates of C. jejuni from nonhuman sources (chicken, swine, etc.), 166 (83%) were typable, 145 cultures agglutinated in various single antisera, and 21 strains agglutinated with different pairs of antisera. Among isolates from all sources, 8 serogroups (1, 2, 4, 5, 7, 8, 9, and 11) were encountered most frequently. Serogroups 1, 2, 4, 5, 7, 9, and 11 were most common among human isolates; the majority of the chicken and all of the swine isolates belonged to the same serogroups identified from human cases. Very good serological correlation was obtained in 20 family outbreaks and 4 community outbreaks.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott J. D., Dale B., Eldridge J., Jones D. M., Sutcliffe E. M. Serotyping of Campylobacter jejuni/coli. J Clin Pathol. 1980 Aug;33(8):762–766. doi: 10.1136/jcp.33.8.762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arko R. J., Wong K. H., Peacock W. L. Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae. J Clin Microbiol. 1979 Apr;9(4):517–519. doi: 10.1128/jcm.9.4.517-519.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg R. L., Jutila J. W., Firehammer B. D. A revised classification of Vibrio fetus. Am J Vet Res. 1971 Jan;32(1):11–22. [PubMed] [Google Scholar]
  4. Blaser M. J., Waldman R. J., Barrett T., Erlandson A. L. Outbreaks of Campylobacter enteritis in two extended families: evidence for person-to-person transmission. J Pediatr. 1981 Feb;98(2):254–257. doi: 10.1016/s0022-3476(81)80652-x. [DOI] [PubMed] [Google Scholar]
  5. Bokkenheuser V. D., Richardson N. J., Bryner J. H., Roux D. J., Schutte A. B., Koornhof H. J., Freiman I., Hartman E. Detection of enteric campylobacteriosis in children. J Clin Microbiol. 1979 Feb;9(2):227–232. doi: 10.1128/jcm.9.2.227-232.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Butzler J. P., Dekeyser P., Detrain M., Dehaen F. Related vibrio in stools. J Pediatr. 1973 Mar;82(3):493–495. doi: 10.1016/s0022-3476(73)80131-3. [DOI] [PubMed] [Google Scholar]
  7. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
  8. CATLIN B. W. Transformation of Neisseria meningitidis by deoxyribonucleates from cells and from culture slime. J Bacteriol. 1960 Apr;79:579–590. doi: 10.1128/jb.79.4.579-590.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cadranel S., Rodesch P., Butzler J. P., Dekeyser P. Enteritis due to "related vibrio" in children. Am J Dis Child. 1973 Aug;126(2):152–155. doi: 10.1001/archpedi.1973.02110190134004. [DOI] [PubMed] [Google Scholar]
  10. Dekeyser P., Gossuin-Detrain M., Butzler J. P., Sternon J. Acute enteritis due to related vibrio: first positive stool cultures. J Infect Dis. 1972 Apr;125(4):390–392. doi: 10.1093/infdis/125.4.390. [DOI] [PubMed] [Google Scholar]
  11. Ferreira M. C., Ribeiro V. L., Ricciardi I. D. Campylobacter, dogs and human enteritis. Vet Rec. 1979 Nov 10;105(19):451–451. doi: 10.1136/vr.105.19.451-a. [DOI] [PubMed] [Google Scholar]
  12. KING E. O. Human infections with Vibrio fetus and a closely related vibrio. J Infect Dis. 1957 Sep-Oct;101(2):119–128. doi: 10.1093/infdis/101.2.119. [DOI] [PubMed] [Google Scholar]
  13. Kosunen T. U., Danielsson D., Kjellander J. Serology of Campylobacter fetus ss. jejuni )"related" campylobacters). Demonstration of strain-specific and interstrain-related antigens by immunoelectrophoresis and co-agglutination. Acta Pathol Microbiol Scand B. 1980 Aug;88(4):207–218. [PubMed] [Google Scholar]
  14. Lauwers S., Vlaes L., Butzler J. P. Campylobacter serotyping a epidemiology. Lancet. 1981 Jan 17;1(8212):158–159. doi: 10.1016/s0140-6736(81)90748-0. [DOI] [PubMed] [Google Scholar]
  15. MORGAN W. J. Studies on the antigenic structure of Vibrio fetus. J Comp Pathol. 1959 Apr;69(2):125–140. doi: 10.1016/s0368-1742(59)80012-6. [DOI] [PubMed] [Google Scholar]
  16. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SMITHIES W. R., GIBBONS N. E. The deoxyribose nucleic acid slime layer of some halophilic bacteria. Can J Microbiol. 1955 Oct;1(8):614–621. doi: 10.1139/m55-074. [DOI] [PubMed] [Google Scholar]
  18. Severin W. P. Campylobacter en enteritis. Ned Tijdschr Geneeskd. 1978 Apr 15;122(15):499–504. [PubMed] [Google Scholar]
  19. Skirrow M. B., Benjamin J. Differentiation of enteropathogenic Campylobacter. J Clin Pathol. 1980 Nov;33(11):1122–1122. doi: 10.1136/jcp.33.11.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Skirrow M. B., Turnbull G. L., Walker R. E., Young S. E. Campylobacter jejuni enteritis transmitted from cat to man. Lancet. 1980 May 31;1(8179):1188–1188. doi: 10.1016/s0140-6736(80)91644-x. [DOI] [PubMed] [Google Scholar]
  22. Svedhem A., Kaijser B. Isolation of Campylobacter jejuni from domestic animals and pets: probable origin of human infection. J Infect. 1981 Mar;3(1):37–40. doi: 10.1016/s0163-4453(81)92261-1. [DOI] [PubMed] [Google Scholar]
  23. Svedhem A., Norkrans G. Campylobacter jejuni enteritis transmitted from cat to man. Lancet. 1980 Mar 29;1(8170):713–714. [PubMed] [Google Scholar]
  24. WHEELER W. E., BORCHERS J. Vibrionic enteritis in infants. Am J Dis Child. 1961 Jan;101:60–66. doi: 10.1001/archpedi.1961.04020020062010. [DOI] [PubMed] [Google Scholar]

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