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. 1982 Jul;16(1):205–208. doi: 10.1128/jcm.16.1.205-208.1982

Identification and typing of herpes simplex viruses with monoclonal antibodies.

N Balachandran, B Frame, M Chernesky, E Kraiselburd, Y Kouri, D Garcia, C Lavery, W E Rawls
PMCID: PMC272324  PMID: 6286719

Abstract

Monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. Complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral DNA and by indirect immunofluorescence with monoclonal antibodies. Examination of a limited number of ulcerative lesions revealed that indirect immunofluorescence and virus isolation were comparable in detecting herpes simplex virus. The results indicate that monoclonal antibodies can be used to accurately identify and type isolates of herpes simplex virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam E., Kaufman R. H., Mirkovic R. R., Melnick J. L. Persistence of virus shedding in asymptomatic women after recovery from herpes genitalis. Obstet Gynecol. 1979 Aug;54(2):171–173. [PubMed] [Google Scholar]
  2. BIEGELEISEN J. Z., Jr, SCOTT L. V., LEWIS V., Jr Rapid diagnosis of herpes simplex virus infections with fluorescent antibody. Science. 1959 Mar 6;129(3349):640–641. doi: 10.1126/science.129.3349.640. [DOI] [PubMed] [Google Scholar]
  3. Balachandran N., Harnish D., Killington R. A., Bacchetti S., Rawls W. E. Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2. J Virol. 1981 Aug;39(2):438–446. doi: 10.1128/jvi.39.2.438-446.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benjamin D. R. Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method. Appl Microbiol. 1974 Oct;28(4):568–571. doi: 10.1128/am.28.4.568-571.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buchman T. G., Roizman B., Adams G., Stover B. H. Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J Infect Dis. 1978 Oct;138(4):488–498. doi: 10.1093/infdis/138.4.488. [DOI] [PubMed] [Google Scholar]
  6. Forghani B., Schmidt N. J., Lennette E. H. Solid phase radioimmunoassay for identification of Herpesvirus hominis types 1 and 2 from clinical materials. Appl Microbiol. 1974 Oct;28(4):661–667. doi: 10.1128/am.28.4.661-667.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gardner P. S., McQuillin J., Black M. M., Richardson J. Rapid diagnosis of herpesvirus hominis infections in superficial lesions by immunofluorescent antibody technics. Br Med J. 1968 Oct 12;4(5623):89–92. doi: 10.1136/bmj.4.5623.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guinan M. E., MacCalman J., Kern E. R., Overall J. C., Jr, Spruance S. L. The course of untreated recurrent genital herpes simplex infection in 27 women. N Engl J Med. 1981 Mar 26;304(13):759–763. doi: 10.1056/NEJM198103263041305. [DOI] [PubMed] [Google Scholar]
  9. Hitchcock G., Randell P. L., Wishart M. M. Herpes simplex lesions of the skin diagnosed by the immunofluorescence technique. Med J Aust. 1974 Aug 24;2(8):280–284. doi: 10.5694/j.1326-5377.1974.tb70771.x. [DOI] [PubMed] [Google Scholar]
  10. Jeansson S. Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis. Appl Microbiol. 1972 Jul;24(1):96–100. doi: 10.1128/am.24.1.96-100.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KAUFMAN H. E. The diagnosis of corneal herpes simplex infection by fluorescent antibody staining. Arch Ophthalmol. 1960 Sep;64:382–384. doi: 10.1001/archopht.1960.01840010384009. [DOI] [PubMed] [Google Scholar]
  12. Kalinyak J. E., Fleagle G., Docherty J. J. Incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women. J Med Virol. 1977;1(3):175–181. doi: 10.1002/jmv.1890010304. [DOI] [PubMed] [Google Scholar]
  13. Kaufman R. H., Gardner H. L., Rawls W. E., Dixon R. E., Young R. L. Clinical features of herpes genitalis. Cancer Res. 1973 Jun;33(6):1446–1451. [PubMed] [Google Scholar]
  14. Killington R. A., Newhook L., Balachandran N., Rawls W. E., Bacchetti S. Production of hybrid cell lines secreting antibodies to herpes simplex virus type 2. J Virol Methods. 1981 Mar;2(4):223–236. doi: 10.1016/0166-0934(81)90012-4. [DOI] [PubMed] [Google Scholar]
  15. Lonsdale D. M. A rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. Lancet. 1979 Apr 21;1(8121):849–852. doi: 10.1016/s0140-6736(79)91265-0. [DOI] [PubMed] [Google Scholar]
  16. Lowry S. P., Melnick J. L., Rawls W. E. Investigation of plaque formation in chick embryo cells as a biological marker for distinguishing herpes virus type 2 from type 1. J Gen Virol. 1971 Jan;10(1):1–9. doi: 10.1099/0022-1317-10-1-1. [DOI] [PubMed] [Google Scholar]
  17. Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nahmias A. J., Naib Z. M., Josey W. E., Clepper A. C. Genital herpes simplex infection. Virologic and cytologic studies. Obstet Gynecol. 1967 Mar;29(3):395–400. [PubMed] [Google Scholar]
  19. Nahmias A. J., Roizman B. Infection with herpes-simplex viruses 1 and 2. 1. N Engl J Med. 1973 Sep 27;289(13):667–674. doi: 10.1056/NEJM197309272891305. [DOI] [PubMed] [Google Scholar]
  20. Nahmias A., DelBuono I., Pipkin J., Hutton R., Wickliffe C. Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique. Appl Microbiol. 1971 Sep;22(3):455–458. doi: 10.1128/am.22.3.455-458.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ng A. B., Reagan J. W., Yen S. S. Hepres genitalis; clinical and cytopathologic experience with 256 patients. Obstet Gynecol. 1970 Oct;36(4):645–651. [PubMed] [Google Scholar]
  22. Nordlund J. J., Anderson C., Hsiung G. D., Tenser R. B. The use of temperature sensitivity and selective cell culture systems for differentiation of herpes simplex virus types 1 and 2 in a clinical laboratory. Proc Soc Exp Biol Med. 1977 May;155(1):118–123. doi: 10.3181/00379727-155-39757. [DOI] [PubMed] [Google Scholar]
  23. Olding-Stenkvist E., Grandien M. Early diagnosis of virus-caused vesicular rashes by immunofluorescence on skin biopsies. I. Varicella, zoster and herpes simplex. Scand J Infect Dis. 1976;8(1):27–35. [PubMed] [Google Scholar]
  24. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  25. Rawls W. E., Gardner H. L., Flanders R. W., Lowry S. P., Kaufman R. H., Melnick J. L. Genital herpes in two social groups. Am J Obstet Gynecol. 1971 Jul 1;110(5):682–689. doi: 10.1016/0002-9378(71)90254-7. [DOI] [PubMed] [Google Scholar]
  26. Rubin S. J., Wende R. D., Rawls W. E. Direct immunofluorescence test for the diagnosis of genital herpesvirus infections. Appl Microbiol. 1973 Sep;26(3):373–375. doi: 10.1128/am.26.3.373-375.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Spruance S. L., Overall J. C., Jr, Kern E. R., Krueger G. G., Pliam V., Miller W. The natural history of recurrent herpes simplex labialis: implications for antiviral therapy. N Engl J Med. 1977 Jul 14;297(2):69–75. doi: 10.1056/NEJM197707142970201. [DOI] [PubMed] [Google Scholar]
  28. Taber L. H., Brasier F., Couch R. B., Greenberg S. B., Jones D., Knight V. Diagnosis of herpes simplex virus infection by immunofluorescence. J Clin Microbiol. 1976 Mar;3(3):309–312. doi: 10.1128/jcm.3.3.309-312.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tada A., Sekine N., Toba M., Yoshino K. An analysis of factors influencing the isolation rate of herpes simplex virus. Microbiol Immunol. 1977;21(4):219–229. doi: 10.1111/j.1348-0421.1977.tb00283.x. [DOI] [PubMed] [Google Scholar]
  30. Tomlinson A. H., Chinn I. J., MacCallum F. O. Immunofluorescence staining for the diagnosis of herpes encephalitis. J Clin Pathol. 1974 Jun;27(6):495–499. doi: 10.1136/jcp.27.6.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Veien N. K., Vestergaard B. F. Rapid diagnostic tests for cutaneous eruptions of herpes simplex. Acta Derm Venereol. 1978;58(1):83–85. [PubMed] [Google Scholar]
  32. Vestergaard B. F., Thybo H. Rapid diagnosis and typind of herpesvirus hominis by the fluorescent antibody method. Scand J Infect Dis. 1974;6(2):113–116. doi: 10.3109/inf.1974.6.issue-2.03. [DOI] [PubMed] [Google Scholar]

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