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. 1982 Aug;16(2):377–381. doi: 10.1128/jcm.16.2.377-381.1982

Potassium tartrate-glycerol as a density gradient substrate for separation of small, round viruses from human feces.

C R Ashley, E O Caul
PMCID: PMC272364  PMID: 6288767

Abstract

Cesium chloride density gradients are frequently used for virus concentration or purification in the preparation of human feces for examination by electron microscopy, Disruption of some of the fecal viruses occurs if they are pelleted from the density gradient in an additional concentration step. This report highlights an important limitation imposed by the use of cesium chloride as a density gradient substrate in attempting to recover small, round, virus-like particles from feces and suggests an alternative substrate which preserves virus morphology without the use of additional protective agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton H., Buckley M., Thom B. T., Cotton J. L., Henderson S. Virus-like particles in winter vomiting disease. Lancet. 1977 Feb 19;1(8008):409–411. doi: 10.1016/s0140-6736(77)92614-9. [DOI] [PubMed] [Google Scholar]
  2. Appleton H., Pereira M. S. A possible virus aetiology in outbreaks of food-poisoning from cockles. Lancet. 1977 Apr 9;1(8015):780–781. doi: 10.1016/s0140-6736(77)92960-9. [DOI] [PubMed] [Google Scholar]
  3. Ashley C. R., Caul E. O., Paver W. K. Astrovirus-associated gastroenteritis in children. J Clin Pathol. 1978 Oct;31(10):939–943. doi: 10.1136/jcp.31.10.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caul E. O., Ashley C., Pether J. V. "Norwalk"-like particles in the epidemic gastroenteritis in the U.K. Lancet. 1979 Dec 15;2(8155):1292–1292. doi: 10.1016/s0140-6736(79)92295-5. [DOI] [PubMed] [Google Scholar]
  5. Christopher P. J., Grohmann G. S., Millson R. H., Murphy A. M. Parvovirus gastroenteritis--a new entity for Australia. Med J Aust. 1978 Feb 11;1(3):121–124. doi: 10.5694/j.1326-5377.1978.tb107781.x. [DOI] [PubMed] [Google Scholar]
  6. Cubitt W. D., McSwiggan D. A., Moore W. Winter vomiting disease caused by calicivirus. J Clin Pathol. 1979 Aug;32(8):786–793. doi: 10.1136/jcp.32.8.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kogasaka R., Sakuma Y., Chiba S., Akihara M., Horino K., Nakao T. Small round virus-like particles associated with acute gastroenteritis in Japanese children. J Med Virol. 1980;5(2):151–160. doi: 10.1002/jmv.1890050209. [DOI] [PubMed] [Google Scholar]
  9. Kurtz J. B., Lee T. W., Pickering D. Astrovirus associated gastroenteritis in a children's ward. J Clin Pathol. 1977 Oct;30(10):948–952. doi: 10.1136/jcp.30.10.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Madeley C. R., Cosgrove B. P., Bell E. J., Fallon R. J. Stool viruses in babies in Glasgow. I. Hospital admissions with diarrhoea. J Hyg (Lond) 1977 Apr;78(2):261–273. doi: 10.1017/s0022172400056151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
  12. Obijeski J. F., Marchenko A. T., Bishop D. H., Cann B. W., Murphy F. A. Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. J Gen Virol. 1974 Jan;22(1):21–33. doi: 10.1099/0022-1317-22-1-21. [DOI] [PubMed] [Google Scholar]
  13. Paver W. K., Ashley C. R., Caul E. O., Clarke S. K. A small virus in human faeces. Lancet. 1973 Feb 3;1(7797):237–240. doi: 10.1016/s0140-6736(73)90072-x. [DOI] [PubMed] [Google Scholar]
  14. Paver W. K., Caul E. O., Clarke S. K. Comparison of 22 nm virus from human faeces with animal parvoviruses. J Gen Virol. 1974 Mar;22(3):447–450. doi: 10.1099/0022-1317-22-3-447. [DOI] [PubMed] [Google Scholar]
  15. Taniguchi K., Urasawa S., Urasawa T. Virus-like particle, 35 to 40 nm, associated with an institutional outbreak of acute gastroenteritis in adults. J Clin Microbiol. 1979 Nov;10(5):730–736. doi: 10.1128/jcm.10.5.730-736.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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