Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1982 Dec;16(6):1123–1126. doi: 10.1128/jcm.16.6.1123-1126.1982

Demonstration of age-dependent capsular material on Pasteurella haemolytica serotype 1.

R E Corstvet, M J Gentry, P R Newman, J A Rummage, A W Confer
PMCID: PMC272551  PMID: 6186688

Abstract

Extracellular capsular material was demonstrated on early log-phase cells of Pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. The presence of this material was shown to be age dependent. Wide capsules were demonstrable on cells from 2- to 12-h cultures, whereas cells from 16- to 22-h cultures had very little cell-associated capsular material. The Maneval technique most clearly demonstrated the presence of capsules on cells from young (6-h) cultures when compared with other capsule staining techniques.

Full text

PDF
1125

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIBERSTEIN E. L., GILLS M., KNIGHT H. Serological types of Pasteurella hemolytica. Cornell Vet. 1960 Jul;50:283–300. [PubMed] [Google Scholar]
  2. BIBERSTEIN E. L., MEYER M. E., KENNEDY P. C. Colonial variation of Pasteurella haemolytica isolated from sheep. J Bacteriol. 1958 Oct;76(4):445–452. doi: 10.1128/jb.76.4.445-452.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARTER G. R. A serological study of Pasteurella haemolytica. Can J Microbiol. 1956 Aug;2(5):483–488. doi: 10.1139/m56-058. [DOI] [PubMed] [Google Scholar]
  4. DUGUID J. P., WILKINSON J. F. The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J Gen Microbiol. 1953 Oct;9(2):174–189. doi: 10.1099/00221287-9-2-174. [DOI] [PubMed] [Google Scholar]
  5. Evans H. B., Wells P. W. A mouse model of Pasteurella haemolytica infection and its use in assessment of the efficacy of P haemolytica vaccines. Res Vet Sci. 1979 Sep;27(2):213–217. [PubMed] [Google Scholar]
  6. Frank G. H., Wessman G. E. Rapid plate agglutination procedure for serotyping Pasteurella haemolytica. J Clin Microbiol. 1978 Feb;7(2):142–145. doi: 10.1128/jcm.7.2.142-145.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gentry M. J., Corstvet R. E., Panciera R. J. Extraction of capsular material from Pasteurella haemolytica. Am J Vet Res. 1982 Nov;43(11):2070–2073. [PubMed] [Google Scholar]
  8. Jasmin A. M. An Improved Staining Method for Demonstrating Bacterial Capsules, with Particular Reference to Pasteurella. J Bacteriol. 1945 Sep;50(3):361–363. doi: 10.1128/jb.50.3.361-363.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kume K., Sawata A., Nakase Y. Haemophilus infections in chickens. I. Characterization of Haemophilus paragallinarum isolated from chickens affected with coryza. Nihon Juigaku Zasshi. 1978 Feb;40(1):65–73. doi: 10.1292/jvms1939.40.65. [DOI] [PubMed] [Google Scholar]
  10. Mian F. A., Jarman T. R., Righelato R. C. Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa. J Bacteriol. 1978 May;134(2):418–422. doi: 10.1128/jb.134.2.418-422.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mukkur T. K. Demonstration of cross-protection between Pasteurella multocida type A and Pasteurella haemolytica, serotype 1. Infect Immun. 1977 Dec;18(3):583–585. doi: 10.1128/iai.18.3.583-585.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rehmtulla A. J., Thomson R. G. A review of the lesions in shipping fever of cattle. Can Vet J. 1981 Jan;22(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  13. Schmid E. N., Menge B., Lickfeld K. G. Capsule fine structure in thin slices of Klebsiella pneumoniae Biovar d (Richard). J Ultrastruct Res. 1981 Apr;75(1):41–49. doi: 10.1016/s0022-5320(81)80098-6. [DOI] [PubMed] [Google Scholar]
  14. Troy F. A., 2nd The chemistry and biosynthesis of selected bacterial capsular polymers. Annu Rev Microbiol. 1979;33:519–560. doi: 10.1146/annurev.mi.33.100179.002511. [DOI] [PubMed] [Google Scholar]
  15. Troy F. A. Chemistry and biosynthesis of the poly( -D-glutamyl) capsule in Bacillus licheniformis. I. Properties of the membrane-mediated biosynthetic reaction. J Biol Chem. 1973 Jan 10;248(1):305–315. [PubMed] [Google Scholar]
  16. Wells P. W., Gilmour N. J., Burrells C., Thompson D. A. A serological comparison of Pasteurella haemolytica vaccines containing different adjuvants. Res Vet Sci. 1979 Sep;27(2):248–250. [PubMed] [Google Scholar]
  17. Williams A. G., Wimpenny J. W. Exopolysaccharide production by Pseudomonas NCIB11264 grown in batch culture. J Gen Microbiol. 1977 Sep;102(1):13–21. doi: 10.1099/00221287-102-1-13. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES