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. 1983 Jan;17(1):22–27. doi: 10.1128/jcm.17.1.22-27.1983

Solid-phase enzyme immunoassay for Chlamydial antibodies.

P Saikku, J Paavonen, P Väänänen, A Vaheri
PMCID: PMC272567  PMID: 6338035

Abstract

An enzyme immunoassay (EIA) for chlamydial immunoglobulin G antibodies was developed by using microtiter wells coated with partially purified reticulate bodies of Chlamydia trachomatis serotype L2, grown in McCoy cells, and uninfected McCoy cells as a control. Duplicate testing of a single serum dilution, 1:500, was found to be sufficient. A good correlation between positive reactions was observed in a comparative study of 421 patient sera with the EIA and an inclusion immunofluorescence test. A good correlation between positive reactions was also observed in a comparative study of 140 patient sera with EIA and microimmunofluorescence tests in which chlamydial elementary or reticulate bodies were used as antigens. Sera of 77 healthy control individuals with low titers in inclusion immunofluorescence or complement fixation tests gave negative results in the EIA. Immunoblotting experiments showed that the major antigenic component in the EIA antigen was a protein with an Mr of 39,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen P., Møller B. R. Autoantibodies in patients with acute salpingitis caused by Chlamydia trachomatis. Scand J Infect Dis. 1982;14(1):19–21. doi: 10.3109/inf.1982.14.issue-1.04. [DOI] [PubMed] [Google Scholar]
  2. Bowie W. R., Wang S. P., Alexander E. R., Floyd J., Forsyth P. S., Pollock H. M., Lin J. S., Buchanan T. M., Holmes K. K. Etiology of nongonococcal urethritis. Evidence for Chlamydia trachomatis and Ureaplasma urealyticum. J Clin Invest. 1977 May;59(5):735–742. doi: 10.1172/JCI108694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caldwell H. D., Kromhout J., Schachter J. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun. 1981 Mar;31(3):1161–1176. doi: 10.1128/iai.31.3.1161-1176.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Howard L., Orenstein N. S., King N. W. Purification on renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs. Appl Microbiol. 1974 Jan;27(1):102–106. doi: 10.1128/am.27.1.102-106.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. MATTHIESEN M., VOLKERT M. An ornithosis related antigen from a coccoid bacterium. Acta Pathol Microbiol Scand. 1956;39(2):117–126. doi: 10.1111/j.1699-0463.1956.tb03383.x. [DOI] [PubMed] [Google Scholar]
  7. Richmond S. J., Caul E. O. Fluorescent antibody studies in chlamydial infections. J Clin Microbiol. 1975 Apr;1(4):345–352. doi: 10.1128/jcm.1.4.345-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Riski H., Pyrhönen S., Wager O., Penttinen K. Lack of measurable complement fixing antibodies against viral antigens. Acta Pathol Microbiol Scand B. 1977 Apr;85(2):167–173. doi: 10.1111/j.1699-0463.1977.tb01691.x. [DOI] [PubMed] [Google Scholar]
  9. Saikku P. Chlamydial serology. Scand J Infect Dis Suppl. 1982;32:34–37. [PubMed] [Google Scholar]
  10. Saikku P., Paavonen J. Single-antigen immunofluorescence test for chlamydial antibodies. J Clin Microbiol. 1978 Aug;8(2):119–122. doi: 10.1128/jcm.8.2.119-122.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salonen E. M., Vaheri A. Rapid solid-phase enzyme immunoassay for antibodies to viruses and other microbes: effects of polyethylene glycol. J Immunol Methods. 1981;41(1):95–103. doi: 10.1016/0022-1759(81)90277-5. [DOI] [PubMed] [Google Scholar]
  12. Schachter J., Cles L., Ray R., Hines P. A. Failure of serology in diagnosing chlamydial infections of the female genital tract. J Clin Microbiol. 1979 Nov;10(5):647–649. doi: 10.1128/jcm.10.5.647-649.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schramek S., Kazár J., Sádecký E. Serological cross-reactions of lipid A components of lipopolysaccharides isolated from Chlamydia psittaci and Coxiella burnetii. Acta Virol. 1980 May;24(3):224–224. [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]
  16. Yong E. C., Chinn J. S., Caldwell H. D., Kuo C. C. Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence. J Clin Microbiol. 1979 Sep;10(3):351–356. doi: 10.1128/jcm.10.3.351-356.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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