Abstract
The API Staph-Ident system, a commercially available biochemical and chromogenic substrate micromethod, was evaluated as a means for identifying the species and determining the biotypes of human strains of staphylococci routinely encountered in a clinical microbiology laboratory. The species identity of 152 of 188 (80.9%) unique clinical isolates of staphylococci was correctly predicted by this method after 5 h of incubation according to the recommendations of the manufacturer. When results were determined after 24 h of incubation, the overall accuracy of this procedure was 90.4%. The API Staph-Ident system was not an adequate procedure for assessing strain biotypes since the patterns of biochemical reactivity observed with 53 of 54 (98.2%) unique isolates of Staphylococcus aureus were identical. Similarly, 58 of 62 (93.6%) different strains of S. epidermidis yielded the same biochemical profile.
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Selected References
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- Brun Y., Fleurette J., Forey F. Micromethod for biochemical identification of coagulase-negative staphylococci. J Clin Microbiol. 1978 Nov;8(5):503–508. doi: 10.1128/jcm.8.5.503-508.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doern G. V. Evaluation of a commercial latex agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Mar;15(3):416–418. doi: 10.1128/jcm.15.3.416-418.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kloos W. E., Wolfshohl J. F. Identification of Staphylococcus species with the API STAPH-IDENT system. J Clin Microbiol. 1982 Sep;16(3):509–516. doi: 10.1128/jcm.16.3.509-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol. 1971 Apr;21(4):585–587. doi: 10.1128/am.21.4.585-587.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myrick B. A., Ellner P. D. Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Feb;15(2):275–277. doi: 10.1128/jcm.15.2.275-277.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peny J., Buissière J. Microméthode d'identification des bactéries. II. Identification du genre Staphylococcus. Ann Inst Pasteur (Paris) 1970 Jan;118(1):10–18. [PubMed] [Google Scholar]