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. 1983 Mar;17(3):548–551. doi: 10.1128/jcm.17.3.548-551.1983

Simplified method for typing herpes simplex virus by restriction endonuclease analysis.

M Q Arens, E M Swierkosz
PMCID: PMC272683  PMID: 6302132

Abstract

We have developed a simplified method for unambiguously typing herpes simplex virus. The method depends on the production of cell-associated virus at 34 degrees C and subsequently, on the separation of cellular DNA and viral DNA by Dounce homogenization and the removal of nuclei by centrifugation. Viral nucleic acid was prepared from the cytoplasmic fraction and analyzed after restriction endonuclease cleavage. The method obviates the use of radioactive isotopes, and the viral DNA is effectively free of interfering cellular DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin D. R. Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method. Appl Microbiol. 1974 Oct;28(4):568–571. doi: 10.1128/am.28.4.568-571.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchman T. G., Roizman B., Adams G., Stover B. H. Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J Infect Dis. 1978 Oct;138(4):488–498. doi: 10.1093/infdis/138.4.488. [DOI] [PubMed] [Google Scholar]
  3. Cohen G. H., Factor M. N., Ponce de Leon M. Inhibition of herpes simplex virus type 2 replication by thymidine. J Virol. 1974 Jul;14(1):20–25. doi: 10.1128/jvi.14.1.20-25.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geder L., Skinner G. R. Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescent technique. J Gen Virol. 1971 Aug;12(2):179–182. doi: 10.1099/0022-1317-12-2-179. [DOI] [PubMed] [Google Scholar]
  5. HOGGAN M. D., ROIZMAN B. The effect of the temperature of incubation on the formation and release of herpes simplex virus in infected FL cells. Virology. 1959 Aug;8:508–524. doi: 10.1016/0042-6822(59)90052-2. [DOI] [PubMed] [Google Scholar]
  6. Kelman A. D., Capozza F. E., Kibrick S. Differential action of deoxynucleosides on mammalian cell cultures infected with herpes simplex virus types 1 and 2. J Infect Dis. 1975 Apr;131(4):452–455. doi: 10.1093/infdis/131.4.452. [DOI] [PubMed] [Google Scholar]
  7. Lonsdale D. M. A rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. Lancet. 1979 Apr 21;1(8121):849–852. doi: 10.1016/s0140-6736(79)91265-0. [DOI] [PubMed] [Google Scholar]
  8. Marks-Hellman S., Ho M. Use of biological characteristics to type Herpesvirus hominis types 1 and 2 in diagnostic laboratories. J Clin Microbiol. 1976 Mar;3(3):277–280. doi: 10.1128/jcm.3.3.277-280.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mills K. W., Gerlach E. H., Bell J. W., Farkas M. E., Taylor R. J. Serotyping herpes simplex virus isolated by enzyme-linked immunosorbent assays. J Clin Microbiol. 1978 Jan;7(1):73–76. doi: 10.1128/jcm.7.1.73-76.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nahmias A. J., Chiang W. T., Del Buono I., Duffey A. Typing of Herpesvirus hominis strains by a direct immunofluorescent technique. Proc Soc Exp Biol Med. 1969 Oct;132(1):386–390. doi: 10.3181/00379727-132-34221. [DOI] [PubMed] [Google Scholar]
  11. Nahmias A., DelBuono I., Pipkin J., Hutton R., Wickliffe C. Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique. Appl Microbiol. 1971 Sep;22(3):455–458. doi: 10.1128/am.22.3.455-458.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nordlund J. J., Anderson C., Hsiung G. D., Tenser R. B. The use of temperature sensitivity and selective cell culture systems for differentiation of herpes simplex virus types 1 and 2 in a clinical laboratory. Proc Soc Exp Biol Med. 1977 May;155(1):118–123. doi: 10.3181/00379727-155-39757. [DOI] [PubMed] [Google Scholar]
  13. Roizman B., Spear P. G. Preparation of herpes simplex virus of high titer. J Virol. 1968 Jan;2(1):83–84. doi: 10.1128/jvi.2.1.83-84.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Seal L. A., Jamison R. M. A simple method for typing clinical isolates of herpesvirus simplex: replication in the presence of 2H-1,3-oxazine-2,6 (3H)-dione (Oxauracil). J Med Virol. 1980;6(1):21–27. doi: 10.1002/jmv.1890060104. [DOI] [PubMed] [Google Scholar]
  15. Walboomers J. M., Schegget J. T. A new method for the isolation of herpes simplex virus type 2 DNA. Virology. 1976 Oct 1;74(1):256–258. doi: 10.1016/0042-6822(76)90151-3. [DOI] [PubMed] [Google Scholar]
  16. Wentworth B. B., Zablotney S. L. Efficiency of plating on chick embryo cells and kinetic neutralization of Herpesvirus hominis strains. Infect Immun. 1972 Mar;5(3):377–382. doi: 10.1128/iai.5.3.377-382.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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