Abstract
The Sensititre identification system (Seward Laboratory/GIBCO Laboratories) consists of a microplate containing a pattern of 24 biochemicals repeated four times together with an automatic inoculation device and a microcomputer-assisted data interpretation component. A total of 1,415 isolates of Enterobacteriaceae plus 6 isolates of other glucose-fermenting gram-negative bacilli were tested in three hospital laboratories in parallel with API 20E (Analytab Products). Discrepancies were resolved by conventional biochemical testing. Sensititre yielded correct identifications at the species level with 94.6% of the isolates and at the genus level with an additional 1.9%. API 20E yielded correct species identification with 91.1% and genus only identification with an additional 6.7% of the isolates. For the routine identification of clinical Enterobacteriaceae isolates, the Sensititre system compares favorably with API 20E and offers clinical laboratories the economy of a microtiter plate system as well as the benefit of a microcomputer capable of other microbiological and data management applications.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barry A. L., Badal R. E., Effinger L. J. Identification of Enterobacteriaceae in frozen microdilution trays prepared by Micro-Media Systems. J Clin Microbiol. 1979 Oct;10(4):492–496. doi: 10.1128/jcm.10.4.492-496.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly S. A., Washington J. A., 2nd Evaluation of Micro-Media Quad Panels for identification of the Enterobacteriaceae. J Clin Microbiol. 1979 Oct;10(4):515–518. doi: 10.1128/jcm.10.4.515-518.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willcox W. R., Lapage S. P., Holmes B. A review of numerical methods in bacterial identification. Antonie Van Leeuwenhoek. 1980;46(3):233–299. doi: 10.1007/BF00453024. [DOI] [PubMed] [Google Scholar]