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. 1983 May;17(5):787–790. doi: 10.1128/jcm.17.5.787-790.1983

Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test.

K R Mittal, R Higgins, S Lariviere
PMCID: PMC272741  PMID: 6190838

Abstract

Serological properties of antigens extracted from strains of Haemophilus pleuropneumoniae belonging to seven different serotypes were investigated. Antisera were prepared in rabbits against Formalin-treated whole cell suspensions as well as autoclaved cell suspensions. Saline and heat extracts and their alcohol precipitate antigens of H. pleuropneumoniae were used in the indirect hemagglutination test. All the antigens used were easily adsorbed directly onto sheep erythrocytes. Saline extract antigen showed maximum type specificity. Heating of the whole cell suspension revealed the cross-reactive minor antigenic determinants. Thus, the heat extract preparations had both type-specific and species-specific antigens. It is suggested that the indirect hemagglutination test may be useful for both serotyping and serodiagnosis of H. pleuropneumoniae infections in pigs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIBERSTEIN E. L., GILLS M., KNIGHT H. Serological types of Pasteurella hemolytica. Cornell Vet. 1960 Jul;50:283–300. [PubMed] [Google Scholar]
  2. Biberstein E. L., Gunnarsson A., Hurvell B. Cultural and biochemical criteria for the identification of haemophilus spp from swine. Am J Vet Res. 1977 Jan;38(1):7–11. [PubMed] [Google Scholar]
  3. CARTER G. R. Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types. Am J Vet Res. 1955 Jul;16(60):481–484. [PubMed] [Google Scholar]
  4. Greenway J. A. Hemophilus pneumonia in B.C. swine. Can Vet J. 1981 Jan;22(1):20–21. [PMC free article] [PubMed] [Google Scholar]
  5. Gunnarsson A., Biberstein E. L., Hurvell B. Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): agglutination reactions. Am J Vet Res. 1977 Aug;38(8):1111–1114. [PubMed] [Google Scholar]
  6. Gunnarsson A. Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): extraction of type-specific antigens. Am J Vet Res. 1979 Apr;40(4):469–472. [PubMed] [Google Scholar]
  7. Mittal K. R., Higgins R., Lariviere S. Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniae. J Clin Microbiol. 1982 Jun;15(6):1019–1023. doi: 10.1128/jcm.15.6.1019-1023.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nicolet J. Sur l'hémophilose du pore. 3. Différenciation sérologique de Haemophilus parahaemolyticus. Zentralbl Bakteriol Orig. 1971;216(4):487–495. [PubMed] [Google Scholar]
  10. Nielsen R., Mandrup M. Pleuropneumonia in swine caused by Haemophilus parahaemolyticus. A study of the epidemiology of the infection. Nord Vet Med. 1977 Nov;29(11):465–473. [PubMed] [Google Scholar]
  11. Nielsen R. Serological and immunological studies of pleuropneumonia of swine caused by Haemophilus parahaemolyticus. Acta Vet Scand. 1974;15(1):80–89. doi: 10.1186/BF03547495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosendal S., Boyd D. A. Haemophilus pleuropneumoniae serotyping. J Clin Microbiol. 1982 Nov;16(5):840–843. doi: 10.1128/jcm.16.5.840-843.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rosendal S., Lombin L., DeMoor J. Serotyping and detection of Haemophilus pleuropneumoniae by indirect fluorescent antibody technique. Can J Comp Med. 1981 Jul;45(3):271–274. [PMC free article] [PubMed] [Google Scholar]
  14. Schiefer B., Moffatt R. E., Greenfield J., Agar J. L., Majka J. A. Porcine Hemophilus parahemolyticus pneumonia in Saskatchewan. I. Natural occurrence and findings. Can J Comp Med. 1974 Apr;38(2):99–104. [PMC free article] [PubMed] [Google Scholar]
  15. Sochard M. R., Colwell R. R. Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination. J Clin Microbiol. 1979 Dec;10(6):890–896. doi: 10.1128/jcm.10.6.890-896.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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