Abstract
Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal extracts. The antigen(s) responsible for this cross-reactivity was partially purified by Sephacryl S-200 gel chromatography and analyzed by fused rocket immunoelectrophoresis. Further analysis by immunodiffusion showed that probably two antigens in the gel chromatography-consolidated fractions were common to the S. uberis and group B and E isolates, but that one of the antigens present was unique to S. uberis. Trypsin destroyed the immunoreactivity of this antigen. Isolation of this common antigen could possibly alleviate some of the tedium associated with the identification of this organism.
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- Baker C. J., Kasper D. L., Davis C. E. Immunochemical characterization of the "native" type III polysaccharide of group B Streptococcus. J Exp Med. 1976 Feb 1;143(2):258–270. doi: 10.1084/jem.143.2.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker C. J., Kasper D. L. Microcapsule of type III strains of group B Streptococcus: production and morphology. Infect Immun. 1976 Jan;13(1):189–194. doi: 10.1128/iai.13.1.189-194.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crowle A. J., Cline L. J. An improved stain for immunodiffusion tests. J Immunol Methods. 1977;17(3-4):379–381. doi: 10.1016/0022-1759(77)90122-3. [DOI] [PubMed] [Google Scholar]
- Harrington J. C., Fenton J. W., 2nd, Pert J. H. Polymer-induced precipitation of antigen-antibody complexes: "precipiplex" reactions. Immunochemistry. 1971 May;8(5):413–421. doi: 10.1016/0019-2791(71)90504-0. [DOI] [PubMed] [Google Scholar]
- Knox K. W., Wicken A. J. Immunological properties of teichoic acids. Bacteriol Rev. 1973 Jun;37(2):215–257. doi: 10.1128/br.37.2.215-257.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roguinsky M. Caractères biochimiques et sérologiques de Streptococcus uberis. Ann Inst Pasteur (Paris) 1971 Feb;120(2):154–163. [PubMed] [Google Scholar]
- Roguinsky M. Réactions de Streptococcus uberis avec les sérums G et P. Ann Inst Pasteur (Paris) 1969 Oct;117(4):529–532. [PubMed] [Google Scholar]
- Shuman R. D., Nord N., Brown R. W., Wessman G. E. Biochemical and serological characteristics of Lancefield groups E, P, and U Streptococci and Streptococcus uberis. Cornell Vet. 1972 Oct;62(4):540–568. [PubMed] [Google Scholar]
- Shuman R. D., Wood R. L., Nord N. Swine abscesses caused by Lancefield's group E streptococci. V. Specificity of the precipitin test for their detection with relation to streptococcal antigens of groups P and U and Corynebacterium pyogenes. Cornell Vet. 1969 Jan;59(1):102–120. [PubMed] [Google Scholar]
- Shuman R. D., Wood R. L., Wessman G. E., Nord N. Swine abscesses caused by Lancefield's group E streptococci. X. Specificity of the precipitin test for their detection with relation to streptococcal antigens of groups R and T, Streptococcus uberis, and Staphylococcus aureus. Cornell Vet. 1972 Jan;62(1):61–73. [PubMed] [Google Scholar]
- Svendsen P. J. Fused rocket immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:69–70. doi: 10.1111/j.1365-3083.1973.tb03781.x. [DOI] [PubMed] [Google Scholar]
- Weeke B. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures. Scand J Immunol Suppl. 1973;1:15–35. doi: 10.1111/j.1365-3083.1973.tb03776.x. [DOI] [PubMed] [Google Scholar]
- Wessman G. E., Shuman R. D. Swine abscesses caused by Lancefield's group E streptococci. XI. Application of the hemagglutination test to demonstrate a correlation between the antigens of group E and streptococcus uberis, and their relation to antibodies induced by unknown organisms. Cornell Vet. 1973 Oct;63(4):618–629. [PubMed] [Google Scholar]
- van de Rijn I., Zabriskie J. B., McCarty M. Group A streptococcal antigens cross-reactive with myocardium. Purification of heart-reactive antibody and isolation and characterization of the streptococcal antigen. J Exp Med. 1977 Aug 1;146(2):579–599. doi: 10.1084/jem.146.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]