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. 1983 Nov;18(5):1113–1118. doi: 10.1128/jcm.18.5.1113-1118.1983

Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates.

W L Thacker, H W Wilkinson, R F Benson
PMCID: PMC272851  PMID: 6358251

Abstract

It is technically impractical for many clinical laboratories to use the direct immunofluorescence assay for identifying and serogrouping clinical isolates of Legionella. We compared the results obtained with the direct immunofluorescence assay with the results of a simple and less-demanding slide agglutination test for identifying 15 serogroups representing seven Legionella species. The slide agglutination test was in complete agreement with the direct immunofluorescence assay, and the serogroup to which 64 clinical isolates of Legionella belonged was correctly identified. With polyvalent, pooled antisera and absorbed, serogroup-specific antisera, the slide agglutination test is a useful alternative to the direct immunofluorescence assay in the diagnosis of Legionella infections and for studying the serological relationships of Legionella-like organisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bibb W. F., Arnow P. M., Dellinger D. L., Perryman S. R. Isolation and characterization of a seventh serogroup of Legionella pneumophila. J Clin Microbiol. 1983 Feb;17(2):346–348. doi: 10.1128/jcm.17.2.346-348.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bibb W. F., Sorg R. J., Thomason B. M., Hicklin M. D., Steigerwalt A. G., Brenner D. J., Wulf M. R. Recognition of a second serogroup of Legionella longbeachae. J Clin Microbiol. 1981 Dec;14(6):674–677. doi: 10.1128/jcm.14.6.674-677.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bissett M. L., Lee J. O., Lindquist D. S. New serogroup of Legionella pneumophila, serogroup 8. J Clin Microbiol. 1983 May;17(5):887–891. doi: 10.1128/jcm.17.5.887-891.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brenner D. J., Steigerwalt A. G., McDade J. E. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Intern Med. 1979 Apr;90(4):656–658. doi: 10.7326/0003-4819-90-4-656. [DOI] [PubMed] [Google Scholar]
  5. Cherry W. B., Gorman G. W., Orrison L. H., Moss C. W., Steigerwalt A. G., Wilkinson H. W., Johnson S. E., McKinney R. M., Brenner D. J. Legionella jordanis: a new species of Legionella isolated from water and sewage. J Clin Microbiol. 1982 Feb;15(2):290–297. doi: 10.1128/jcm.15.2.290-297.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cordes L. G., Wilkinson H. W., Gorman G. W., Fikes B. J., Fraser D. W. Atypical Legionella-like organisms: fastidious water-associated bacteria pathogenic for man. Lancet. 1979 Nov 3;2(8149):927–930. doi: 10.1016/s0140-6736(79)92623-0. [DOI] [PubMed] [Google Scholar]
  8. Edelstein P. H., Brenner D. J., Moss C. W., Steigerwalt A. G., Francis E. M., George W. L. Legionella wadsworthii species nova: a cause of human pneumonia. Ann Intern Med. 1982 Dec;97(6):809–813. doi: 10.7326/0003-4819-97-6-809. [DOI] [PubMed] [Google Scholar]
  9. England A. C., 3rd, McKinney R. M., Skaliy P., Gorman G. W. A fifth serogroup of Legionella pneumophila. Ann Intern Med. 1980 Jul;93(1):58–59. doi: 10.7326/0003-4819-93-1-58. [DOI] [PubMed] [Google Scholar]
  10. MAALØE O. The International Reference Preparation for Opacity; notes and description. Bull World Health Organ. 1955;12(5):769–775. [PMC free article] [PubMed] [Google Scholar]
  11. McKinney R. M., Porschen R. K., Edelstein P. H., Bissett M. L., Harris P. P., Bondell S. P., Steigerwalt A. G., Weaver R. E., Ein M. E., Lindquist D. S. Legionella longbeachae species nova, another etiologic agent of human pneumonia. Ann Intern Med. 1981 Jun;94(6):739–743. doi: 10.7326/0003-4819-94-6-739. [DOI] [PubMed] [Google Scholar]
  12. McKinney R. M., Thacker L., Harris P. P., Lewallen K. R., Hebert G. A., Edelstein P. H., Thomason B. M. Four serogroups of Legionnaires' disease bacteria defined by direct immunofluorescence. Ann Intern Med. 1979 Apr;90(4):621–624. doi: 10.7326/0003-4819-90-4-621. [DOI] [PubMed] [Google Scholar]
  13. McKinney R. M., Thomason B. M., Harris P. P., Thacker L., Lewallen K. R., Wilkinson H. W., Hebert G. A., Moss C. W. Recognition of a new serogroup of Legionnaires disease bacterium. J Clin Microbiol. 1979 Jan;9(1):103–107. doi: 10.1128/jcm.9.1.103-107.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McKinney R. M., Wilkinson H. W., Sommers H. M., Fikes B. J., Sasseville K. R., Yungbluth M. M., Wolf J. S. Legionella pneumophila serogroup six: isolation from cases of legionellosis, identification by immunofluorescence staining, and immunological response to infection. J Clin Microbiol. 1980 Sep;12(3):395–401. doi: 10.1128/jcm.12.3.395-401.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morris G. K., Steigerwalt A., Feeley J. C., Wong E. S., Martin W. T., Patton C. M., Brenner D. J. Legionella gormanii sp. nov. J Clin Microbiol. 1980 Nov;12(5):718–721. doi: 10.1128/jcm.12.5.718-721.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Orrison L. H., Cherry W. B., Tyndall R. L., Fliermans C. B., Gough S. B., Lambert M. A., McDougal L. K., Bibb W. F., Brenner D. J. Legionella oakridgensis: unusual new species isolated from cooling tower water. Appl Environ Microbiol. 1983 Feb;45(2):536–545. doi: 10.1128/aem.45.2.536-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson H. W., Fikes B. J. Detection of cell-associated or soluble antigens of Legionella pneumophila serogroups 1 to 6, Legionella bozemanii, Legionella dumoffii, Legionella gormanii, and Legionella micdadei by staphylococcal coagglutination tests. J Clin Microbiol. 1981 Sep;14(3):322–325. doi: 10.1128/jcm.14.3.322-325.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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