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. 1979 Apr;9(4):493–497. doi: 10.1128/jcm.9.4.493-497.1979

Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.

M H Merson, R B Sack, A K Kibriya, A Al-Mahmood, Q S Adamed, I Huq
PMCID: PMC273061  PMID: 379039

Abstract

Diagnosis of enterotoxigenic Escherichia coli diarrhea was made in 109 adult males with an acute dehydrating cholera-like syndrome in Dacca, Bangladesh, by testing 10 colonies isolated from admission stool specimens for production of heat-labile and heat-stable toxins. Toxin testing of one colony yielded a diagnosis in 92% of the cases, testing of two colonies yielded a diagnosis in 95% of the cases, testing of a pool of 5 colonies yielded a diagnosis in 95% of the cases, and testing of a pool of 10 colonies yielded a diagnosis in 96% of the cases. From stool cultures obtained on subsequent days, toxin testing of individual colonies and pools revealed diminished efficacy of pooling with decreasing numbers of enterotoxin-positive isolates in the pool. To detect the presence of enterotoxigenic E. coli in stools, toxin testing of 5 individual isolates and a pool of 10 colonies was found to be almost as effective as the testing of 10 individual isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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