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. 1979 Jul;10(1):1–7. doi: 10.1128/jcm.10.1.1-7.1979

Preparation of reference antisera for laboratory diagnosis of blastomycosis.

J H Green, W K Harrell, J E Johnson, R Benson
PMCID: PMC273083  PMID: 115894

Abstract

Antiserum was prepared in rabbits against the A antigen and a new antigen (labeled the D antigen) of yeast-phase cells of Blastomyces dermatitidis by using immunoelectrophoretic precipitin arcs as vaccine. The antiserum can be used as a reference serum in the immunodiffusion test for identyfying antibodies to the A antigen in sera from patients thought to have blastomycosis. Antibodies to the D antigen have not yet been found in human sera. The rabbit antiserum that was absorbed with Histoplasma capsulatum yeast-phase cells did not react in the complement fixation test with either H. capsulatum yeast-phase or mycelial-phase complement fixation antigen. Therefore, it could also be used as a positive control serum in the complement fixation test. A fluorescent-antibody conjugate prepared from the absorbed serum was found to be valuable for the identification of yeast-phase cells of B. dermatitidis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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