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. 1979 Aug;10(2):168–174. doi: 10.1128/jcm.10.2.168-174.1979

Biotypes of Haemophilus encountered in clinical laboratories.

T R Oberhofer, A E Back
PMCID: PMC273122  PMID: 315964

Abstract

The biochemical characteristics of 464 strains of Haemophilus influenzae and 83 strains of Haemophilus parainfluenzae isolated over an 18-month period are described. Of 22 characteristics obtained, only 6 were necessary to biochemically identify and biotype the isolates. The key substrates or tests were urease, ornithine, indole, o-nitrophenyl-beta-D-galactopyranoside, sucrose, and xylose. Five biotypes of H. influenzae and four of H. parainfluenzae were commonly recognized. Some strains were encountered which could not be accommodated in the recognized taxa but which constituted separate biotypes of the two species, H. influenzae biotype I was recovered principally from blood, cerebrospinal fluid, and upper respiratory secretion, and biotypes II and III were recovered from eye and sputum cultures. Biotype I was recovered primarily from children less than 1 year of age, whereas biotypes II and III were from persons 1 to 5 years old and from those over 20 years of age. Multiple isolates recovered from the same patient were almost always of the same biotype. Strains of H. parainfluenzae were isolated primarily from sputum, with others being isolated from body sources such as dental abscesses, gastric aspirates, and peritoneal fluid. An inverse relationship was noticed between hemolysis and mannose fermentation among H. parainfluenzae biotype III strains, whereas the relationship was absent among the other biotypes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albritton W. L., Penner S., Slaney L., Brunton J. Biochemical characteristics of Haemophilus influenzae in relationship to source of isolation and antibiotic resistance. J Clin Microbiol. 1978 Jun;7(6):519–523. doi: 10.1128/jcm.7.6.519-523.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Back A. E., Oberhofer T. R. Use of the Minitek system for biotyping Haemophilus species. J Clin Microbiol. 1978 Mar;7(3):312–313. doi: 10.1128/jcm.7.3.312-313.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bone F. J. Meningitis due to an unusual type of Haemophilus influenzae. Scott Med J. 1975 Jan;20(1):19–21. doi: 10.1177/003693307502000104. [DOI] [PubMed] [Google Scholar]
  4. Buck L. L., Douglas G. W. Meningitis due to Haemophilus influenzae type e. J Clin Microbiol. 1976 Oct;4(4):381–381. doi: 10.1128/jcm.4.4.381-381.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cawthern T. H., Marraro R. V., McCleskey F. K. Bacteremia associated with Haemophilus influenzae type e biotype 4. J Clin Microbiol. 1978 Jan;7(1):1–2. doi: 10.1128/jcm.7.1.1-2.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dahlgren J., Tally F. P., Brothers G., Ruskin J. Haemophilus parainfluenzae endocarditis. Am J Clin Pathol. 1974 Nov;62(5):607–611. doi: 10.1093/ajcp/62.5.607. [DOI] [PubMed] [Google Scholar]
  7. Evans N. M., Bell S. M., Smith D. D. New satellitism test for isolation and identification of Haemophilus influenzae and Haemophilus parainfluenzae in sputum. J Clin Microbiol. 1975 Jan;1(1):89–95. doi: 10.1128/jcm.1.1.89-95.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans N. M., Smith D. D. The effect of the medium and source of growth factors on the satellitism test for Haemophilus species. J Med Microbiol. 1972 Nov;5(4):509–514. doi: 10.1099/00222615-5-4-509. [DOI] [PubMed] [Google Scholar]
  9. Everett E. D., Rham A. E., Jr, Adaniya R., Stevens D. L., McNitt T. R. Haemophilus influenzae pneumonia in adults. JAMA. 1977 Jul 25;238(4):319–321. [PubMed] [Google Scholar]
  10. Hable K. A., Logan G. B., Washington J. A., 2nd Three Hemophilus species. Pathogenic activity. Am J Dis Child. 1971 Jan;121(1):35–37. doi: 10.1001/archpedi.1971.02100120071008. [DOI] [PubMed] [Google Scholar]
  11. Jones R. N., Slepack J., Bigelow J. Ampicillin-resistant Haemophilus paraphrophilus laryngo-epiglottitis. J Clin Microbiol. 1976 Nov;4(5):405–407. doi: 10.1128/jcm.4.5.405-407.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kilian M. A rapid method for the differentiation of Haemophilus strains. The porphyrin test;. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):835–842. doi: 10.1111/j.1699-0463.1974.tb02381.x. [DOI] [PubMed] [Google Scholar]
  13. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  14. Kilian M., Schiott C. R. Haemophili and related bacteria in the human oral cavity. Arch Oral Biol. 1975 Dec;20(12):791–796. doi: 10.1016/0003-9969(75)90055-2. [DOI] [PubMed] [Google Scholar]
  15. Kilian M. The haemolytic activity of Haemophilus species. Acta Pathol Microbiol Scand B. 1976 Dec;84B(6):339–341. doi: 10.1111/j.1699-0463.1976.tb01950.x. [DOI] [PubMed] [Google Scholar]
  16. LOWE M. B. HAEMOPHILUS INFLUENZAE TYPE-C BRONCHOPNEUMONIA. J Pathol Bacteriol. 1964 Jul;88:315–316. doi: 10.1002/path.1700880142. [DOI] [PubMed] [Google Scholar]
  17. Marraro R. V., McCleskey F. K., Mitchell J. L. Pneumonia due to Haemophilus influenzae (H. aegyptius) biotype 3. J Clin Microbiol. 1977 Aug;6(2):172–173. doi: 10.1128/jcm.6.2.172-173.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PITTMAN M., DAVIS D. J. Identification of the Koch-Weeks bacillus (Hemophilus aegyptius). J Bacteriol. 1950 Mar;59(3):413–426. doi: 10.1128/jb.59.3.413-426.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pickering L. K., Simon F. A. Reevaluation of neonatal Hemophilus influenzae infections. South Med J. 1977 Feb;70(2):205-7, 212. doi: 10.1097/00007611-197702000-00031. [DOI] [PubMed] [Google Scholar]
  20. Reddy C. M., Rao K. S., Thomas F. E., Andersen E. R. Hemophilus parainfluenzae bacteremia with meningitis. N C Med J. 1978 Mar;39(3):165–166. [PubMed] [Google Scholar]
  21. Sutter V. L., Finegold S. M. Haemophilus aphrophilus infections: clinical and bacteriologic studies. Ann N Y Acad Sci. 1970 Oct 30;174(2):468–487. doi: 10.1111/j.1749-6632.1970.tb45574.x. [DOI] [PubMed] [Google Scholar]
  22. TUNEVALL G. Studies on Haemophilus influenzae; biochemical activities. Acta Pathol Microbiol Scand. 1951;29(4):387–396. doi: 10.1111/j.1699-0463.1951.tb00145.x. [DOI] [PubMed] [Google Scholar]
  23. Wallace R. J., Jr, Musher D. M., Martin R. R. Hemophilus influenzae pneumonia in adults. Am J Med. 1978 Jan;64(1):87–93. doi: 10.1016/0002-9343(78)90182-1. [DOI] [PubMed] [Google Scholar]

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