Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Sep;10(3):285–289. doi: 10.1128/jcm.10.3.285-289.1979

Use of an immunoperoxidase method for identification of Bacteroides fragilis.

P C Hsu, B H Minshew, B L Williams, E S Lennard
PMCID: PMC273153  PMID: 385616

Abstract

An indirect immunoperoxidase (IP) slide test was evaluated for the laboratory identification of Bacteroides fragilis. Antigen-antibody complexes were detected with goat anti-rabbit immunoglobulin G-peroxidase conjugate with 3-amino-9-ethyl-carbazole as the peroxidase substrate. Ninety-one percent of 44 B. fragilis strains tested were IP positive (3+ to 4+ reactions) with greater than or equal to 1:160 dilutions of rabbit antiserum produced against whole cells of B. fragilis ATCC 23745. The antiserum was species specific. No cross-reactions were observed with 35 Bacteroides strains of other species or with a variety of facultative or aerobic gram-negative bacilli. Four B. fragilis strains were IP negative. One of these (VPI 2393) was the deoxyribonucleic acid (DNA) homology group II reference strain. The other three were clinical isolates. IP-negative and representative IP-positive strains were tested for DNA homology with the type strains for DNA homology groups I and II (VPI 2553 and VPI 2393). Two of the three clinical isolates were classified as DNA homology group II, and the remaining strain was classified as a group I. Capsular material known to be unique to B. fragilis was common to both DNA homology groups as indicated by reactions with purified anticapsular antiserum. The IP technique provides a suitable alternative to fluorescent microscopy for the rapid immunological identification of B. fragilis.

Full text

PDF
288

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abshire R. L., Lombard G. L., Dowell V. R., Jr Fluorescent-antibody studies on selected strains of Bacteroides fragilis subspecies fragilis. J Clin Microbiol. 1977 Oct;6(4):425–432. doi: 10.1128/jcm.6.4.425-432.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chow A. W., Montgomerie J. Z., Guze L. B. Parenteral clindamycin therapy for severe anaerobic infections. Arch Intern Med. 1974 Jul;134(1):78–82. [PubMed] [Google Scholar]
  3. Crosa J. H., Brenner D. J., Falkow S. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol. 1973 Sep;115(3):904–911. doi: 10.1128/jb.115.3.904-911.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fass R. J., Ruiz D. E., Gardner W. G., Rotilie C. A. Clindamycin and gentamicin for aerobic and anaerobic sepsis. Arch Intern Med. 1977 Jan;137(1):28–38. [PubMed] [Google Scholar]
  5. Finegold S. M., Bartlett J. G., Chow A. W., Flora D. J., Gorbach S. L., Harder E. J., Tally F. P. Management of anaerobic infections. Ann Intern Med. 1975 Sep;83(3):375–389. doi: 10.7326/0003-4819-83-3-375. [DOI] [PubMed] [Google Scholar]
  6. Griffin M. H. Fluorescent antibody techniques in the identification of the gram-negative nonsporeforming anaerobes. Health Lab Sci. 1970 Apr;7(2):78–83. [PubMed] [Google Scholar]
  7. Kasper D. L., Hayes M. E., Reinap B. G., Craft F. O., Onderdonk A. B., Polk B. F. Isolation and identification of encapsulated strains of Bacteroides fragilis. J Infect Dis. 1977 Jul;136(1):75–81. doi: 10.1093/infdis/136.1.75. [DOI] [PubMed] [Google Scholar]
  8. Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
  9. Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
  10. Lambe D. W., Jr, Moroz D. A. Serogrouping of Bacteroides fragilis subsp. fragilis by the agglutination test. J Clin Microbiol. 1976 Jun;3(6):586–592. doi: 10.1128/jcm.3.6.586-592.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stauffer L. R., Hill E. O., Holland J. W., Altemeier W. A. Indirect fluorescent antibody procedure for the rapid detection and identification of Bacteroides and Fusobacterium in clinical specimens. J Clin Microbiol. 1975 Oct;2(4):337–344. doi: 10.1128/jcm.2.4.337-344.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stone H. H., Kolb L. D., Geheber C. E. Incidence and significance of intraperitoneal anaerobic bacteria. Ann Surg. 1975 May;181(5):705–715. doi: 10.1097/00000658-197505000-00027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Swenson R. M., Lorber B., Michaelson T. C., Spaulding E. H. The bacteriology of intra-abdominal infections. Arch Surg. 1974 Sep;109(3):398–399. doi: 10.1001/archsurg.1974.01360030050013. [DOI] [PubMed] [Google Scholar]
  15. Wilson W. R., Martin W. J., Wilkowske C. J., Washington J. A., 2nd Anaerobic bacteremia. Mayo Clin Proc. 1972 Sep;47(9):639–646. [PubMed] [Google Scholar]
  16. Yunis A. A., Smith U. S., Restrepo A. Reversible bone marrow suppression from chloramphenicol. A consequence of mitochondrial injury. Arch Intern Med. 1970 Aug;126(2):272–275. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES