Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1979 Oct;10(4):464–476. doi: 10.1128/jcm.10.4.464-476.1979

Differentiation of Peptococcus and Peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.

M A Lambert, A Y Armfield
PMCID: PMC273198  PMID: 528680

Abstract

Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonomic decision to transfer aerotolerant Peptostreptococcus species to the genus Streptococcus. Because inconsistencies in the results prevented our differentiating Peptococcus prevotii. Peptococcus magnus, and Peptococcus variabilis by GLC, additional strains will have to been examined. These GLC techniques are amenable to routine use; however, for interlaboratory results to be meaningful, the classification and nomenclature of the anaerobic gram-positive cocci should be standardized.

Full text

PDF
466

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amstein C. F., Hartman P. A. Differentiation of some enterococci by gas chromatography. J Bacteriol. 1973 Jan;113(1):38–41. doi: 10.1128/jb.113.1.38-41.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brian B. L., Gracy R. W., Scholes V. E. Gas chromatography of cyclopropane fatty acid methylesters prepared with methanolic boron trichloride and boron trifluoride. J Chromatogr. 1972 Mar 22;66(1):138–140. doi: 10.1016/s0021-9673(01)82938-1. [DOI] [PubMed] [Google Scholar]
  3. DEIBEL R. H. THE GROUP D STREPTOCOCCI. Bacteriol Rev. 1964 Sep;28:330–366. doi: 10.1128/br.28.3.330-366.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. doi: 10.1128/am.23.6.1131-1139.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graves M. H., Morello J. A., Kocka F. E. Sodium polyanethol sulfonate sensitivity of anaerobic cocci. Appl Microbiol. 1974 Jun;27(6):1131–1133. doi: 10.1128/am.27.6.1131-1133.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holland J. W., Gagnet S. M., Lewis S. A., Stauffer L. R. Clinical evaluation of a simple, rapid procedure for the presumptive identification of anaerobic bacteria. J Clin Microbiol. 1977 Apr;5(4):416–426. doi: 10.1128/jcm.5.4.416-426.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holland J. W., Hill E. O., Altemeier W. A. Numbers and types of anaerobic bacteria isolated from clinical specimens since 1960. J Clin Microbiol. 1977 Jan;5(1):20–25. doi: 10.1128/jcm.5.1.20-25.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lambert M. A., Moss C. W. Gas-liquid chromatography of short-chain fatty acids on Dexsil 300 GC. J Chromatogr. 1972 Dec 20;74(2):335–338. doi: 10.1016/s0021-9673(01)86164-1. [DOI] [PubMed] [Google Scholar]
  9. Lambert M. A., Moss C. W. Use of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria. Appl Microbiol. 1973 Oct;26(4):517–520. doi: 10.1128/am.26.4.517-520.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lambert M. S., Moss C. W. Degradation of bacterial cyclopropane acids with boron trihalide reagents. Microbios. 1977;18(71):51–58. [PubMed] [Google Scholar]
  11. Markowitz A., Lerner A. M. Differentiation of several isolates of Peptococcus magnus by counterimmunoelectrophoresis. Infect Immun. 1977 Apr;16(1):152–154. doi: 10.1128/iai.16.1.152-154.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moss C. W., Dees S. B. Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J Chromatogr. 1975 Oct 29;112:594–604. doi: 10.1016/s0021-9673(00)99988-6. [DOI] [PubMed] [Google Scholar]
  13. Moss C. W., Lambert M. A., Lombard G. L. Cellular fatty acids of Peptococcus variabilis and Peptostreptococcus anaerobius. J Clin Microbiol. 1977 Jun;5(6):665–667. doi: 10.1128/jcm.5.6.665-667.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Porschen R. K., Spaulding E. H. Fluorescent antibody study of the gram-positive anaerobic cocci. Appl Microbiol. 1974 Nov;28(5):851–855. doi: 10.1128/am.28.5.851-855.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wells C. L., Field C. R. Long-chain fatty acids of peptococci and peptostreptococci. J Clin Microbiol. 1976 Dec;4(6):515–521. doi: 10.1128/jcm.4.6.515-521.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES