Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Mar;11(3):213–216. doi: 10.1128/jcm.11.3.213-216.1980

Detection of Salmonella typhi D, Vi, and d antigens, by slide coagglutination, in urine from patients with typhoid fever.

R C Rockhill, L W Rumans, M Lesmana, D T Dennis
PMCID: PMC273365  PMID: 7380998

Abstract

Salmonella typhi antigens D, Vi, and d were detected in the urine of 59 out of 61 (97%) bacteriologically confirmed typhoid fever patients by slide coagglutination with monovalent antisera coupled to protein A-rich staphylococci. These antigens were also detected in the urine of an additional 22 patients, 16 of whom subsequently demonstrated seroconversion by S. typhi O antibody agglutination, but from whom the bacterium was not isolated. The remaining 13 patients had negative urine coagglutination results, no isolation of S. typhi from blood or stool specimens, and no demonstration of seroconversion. These results suggest that the method of slide coagglutination of urine can be used to screen patients with suspected typhoid fever with a high degree of reliability. The method may also have potential importance in the diagnosis of typhoid when the bacterium is not isolated.

Full text

PDF
216

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. E., Joseph S. W., Nasution R., Sunoto, Butler T., Van Peenen P. F., Irving G. S., Saroso J. S., Watten R. H. Febrile illnesses resulting in hospital admission: a bacteriological and serological study in Jakarta, Indonesia. Am J Trop Med Hyg. 1976 Jan;25(1):116–121. doi: 10.4269/ajtmh.1976.25.116. [DOI] [PubMed] [Google Scholar]
  2. BOKKENHEUSER V., SMIT P., RICHARDSON N. A CHALLENGE TO THE VALIDITY OF THE VI TEST FOR THE DETECTION OF CHRONIC TYPHOID CARRIERS. Am J Public Health Nations Health. 1964 Sep;54:1507–1513. doi: 10.2105/ajph.54.9.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Danielsson D., Kronvall G. Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-containing staphylococci. Appl Microbiol. 1974 Feb;27(2):368–374. doi: 10.1128/am.27.2.368-374.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edwards E. A., Hilderbrand R. L. Method for identifying Salmonella and Shigella directly from the primary isolation plate by coagglutination of protein A-containing staphylococci sensitized with specific antibody. J Clin Microbiol. 1976 Mar;3(3):339–343. doi: 10.1128/jcm.3.3.339-343.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edwards E. A., Larson G. L. New method of grouping beta-hemolytic streptococci directly on sheep blood agar plates by coagglutination of specifically sensitized protein A-containing staphylococci. Appl Microbiol. 1974 Dec;28(6):972–976. doi: 10.1128/am.28.6.972-976.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ingall D., Sherman J. D. Chloramphenicol. Pediatr Clin North Am. 1968 Feb;15(1):57–72. doi: 10.1016/s0031-3955(16)32088-0. [DOI] [PubMed] [Google Scholar]
  7. Kaye D., Palmieri M., Eyckmans L., Rocha H., Hook E. W. Comparison of bile and trypticase soy broth for isolation Salmonella from blood. Am J Clin Pathol. 1966 Sep;46(3):408–410. doi: 10.1093/ajcp/46.3_ts.408. [DOI] [PubMed] [Google Scholar]
  8. Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
  9. Olcén P., Danielsson D., Kjellander J. The use of protein A-containing staphylococci sensitized with anti-meningococcal antibodies for grouping Neisseria meningitidis and demonstration of meningococcal antigen in cerebrospinal fluid. Acta Pathol Microbiol Scand B. 1975 Aug;83(4):387–396. doi: 10.1111/j.1699-0463.1975.tb00117.x. [DOI] [PubMed] [Google Scholar]
  10. Raj H. Enrichment medium for selection of Salmonella from fish homogenate. Appl Microbiol. 1966 Jan;14(1):12–20. doi: 10.1128/am.14.1.12-20.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schroeder S. A. Interpretation of serologic tests for typhoid fever. JAMA. 1968 Oct 21;206(4):839–840. [PubMed] [Google Scholar]
  12. Thirumoorthi M. C., Dajani A. S. Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection. J Clin Microbiol. 1979 Jan;9(1):28–32. doi: 10.1128/jcm.9.1.28-32.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wallerstein R. O., Condit P. K., Kasper C. K., Brown J. W., Morrison F. R. Statewide study of chloramphenicol therapy and fatal aplastic anemia. JAMA. 1969 Jun 16;208(11):2045–2050. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES