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. 1980 Aug;12(2):264–270. doi: 10.1128/jcm.12.2.264-270.1980

Antibiotic resistance in Enterotoxigenic and non-enterotoxigenic Escherichia coli.

J M DeBoy 2nd, I K Wachsmuth, B R Davis
PMCID: PMC273564  PMID: 7014609

Abstract

Antibiotic disk susceptibility tests were done on 220 strains of Escherichia coli belonging to serotypes reported in the literature to be associated with the production of enterotoxin. A total of 128 (58%) were resistant to one or more antibiotics, sulfa drugs, or chemotherapeutic agents. An analysis of these strains revealed primary, secondary, and tertiary drug resistance patterns that indicated a selective pattern in the formation of multiple drug resistance in E. coli. Resistances to certain antibiotics were more likely to occur in pairs and triads (secondary resistance patterns) that were often combined or coexisted in a single strain of E. coli to produce tertiary drug resistance patterns, conferring drug resistance to five or six different antibiotics. Among enterotoxin-associated serotypes, single and multiple drug resistance was less frequently associated with enterotoxin-produced strains than with strains from the same serotype that were not enterotoxigenic. Within the enterotoxigenic E. coli, single and multiple resistance to antibiotics was more frequent in strains producing only heat-stable enterotoxin (ST) than in strains producing only heat-labile enterotoxin (LT) or both. The number of resistances to different antibiotics per resistant strain averaged approximately 1.4 for LT plus ST or LT strains, and 3.9 for ST strains and nonenterotoxigenic strains. Phenotypic characterization of 170 strains for four usually plasmid-mediated characteristics showed that the number of antibiotics to which a strain was directly resistant varied with the type and number of plasmid-mediated characteristics present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth P. T., Datta N., Hedges R. W., Grinter N. J. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. J Bacteriol. 1976 Mar;125(3):800–810. doi: 10.1128/jb.125.3.800-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen S. N. Transposable genetic elements and plasmid evolution. Nature. 1976 Oct 28;263(5580):731–738. doi: 10.1038/263731a0. [DOI] [PubMed] [Google Scholar]
  3. Cooke E. M. Properties of strains of Escherichia coli isolated from the faeces of patients with ulcerative colitis, patients with acute diarrhoea and normal persons. J Pathol Bacteriol. 1968 Jan;95(1):101–113. doi: 10.1002/path.1700950112. [DOI] [PubMed] [Google Scholar]
  4. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  5. Deneke C. F., Thorne G. M., Gorbach S. L. Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans. Infect Immun. 1979 Oct;26(1):362–368. doi: 10.1128/iai.26.1.362-368.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goebel W., Royer-Pokora B., Lindenmaier W., Bujard H. Plasmids controlling synthesis of hemolysin in Escherichia coli: molecular properties. J Bacteriol. 1974 Jun;118(3):964–973. doi: 10.1128/jb.118.3.964-973.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gottesman M. M., Rosner J. L. Acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5041–5045. doi: 10.1073/pnas.72.12.5041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gyles C., So M., Falkow S. The enterotoxin plasmids of Escherichia coli. J Infect Dis. 1974 Jul;130(1):40–49. doi: 10.1093/infdis/130.1.40. [DOI] [PubMed] [Google Scholar]
  14. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  15. Jorgensen R. A., Berg D. E., Allet B., Reznikoff W. S. Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance. J Bacteriol. 1979 Jan;137(1):681–685. doi: 10.1128/jb.137.1.681-685.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kudoh Y., Zen-Yoji H., Matsushita S., Sakai S., Maruyama T. Outbreaks of acute enteritis due to heat-stable enterotoxin-producing strains of Escherichia coli. Microbiol Immunol. 1977;21(3):175–178. doi: 10.1111/j.1348-0421.1977.tb00278.x. [DOI] [PubMed] [Google Scholar]
  17. McConnell M. M., Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. Transposition of ampicillin resistance to an enterotoxin plasmid in an Escherichia coli strain of human origin. J Bacteriol. 1979 Aug;139(2):346–355. doi: 10.1128/jb.139.2.346-355.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Monti-Bragadin C., Samer L., Rottini G. D., Pani B. The compatibility of Hly factor, a transmissible element which controls alpha-haemolysin production in Escherichia coli. J Gen Microbiol. 1975 Feb;86(2):367–369. doi: 10.1099/00221287-86-2-367. [DOI] [PubMed] [Google Scholar]
  19. Orskov I., Orskov F. Episome-carried surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. J Bacteriol. 1966 Jan;91(1):69–75. doi: 10.1128/jb.91.1.69-75.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Orskov I., Orskov F. Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities. Med Microbiol Immunol. 1977 Jul 18;163(2):99–110. doi: 10.1007/BF02121825. [DOI] [PubMed] [Google Scholar]
  21. Robinson M. K., Bennett P. M., Richmond M. H. Inhibition of TnA translocation by TnA. J Bacteriol. 1977 Jan;129(1):407–414. doi: 10.1128/jb.129.1.407-414.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ryder R. W., Wachsmuth I. K., Buxton A. E., Evans D. G., DuPont H. L., Mason E., Barrett F. F. Infantile diarrhea produced by heat-stable enterotoxigenic Escherichia coli. N Engl J Med. 1976 Oct 14;295(16):849–853. doi: 10.1056/NEJM197610142951601. [DOI] [PubMed] [Google Scholar]
  23. SMITH H. W. The haemolysins of Escherichia coli. J Pathol Bacteriol. 1963 Jan;85:197–211. doi: 10.1002/path.1700850119. [DOI] [PubMed] [Google Scholar]
  24. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sack R. B. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. doi: 10.1146/annurev.mi.29.100175.002001. [DOI] [PubMed] [Google Scholar]
  26. Sack R. B. The epidemiology of diarrhea due to enterotoxigenic Escherichia coli. J Infect Dis. 1978 May;137(5):639–640. doi: 10.1093/infdis/137.5.639. [DOI] [PubMed] [Google Scholar]
  27. Shore E. G., Dean A. G., Holik K. J., Davis B. R. Enterotoxin-producing Escherichia coli and diarrheal disease in adult travelers: a prospective study. J Infect Dis. 1974 May;129(5):577–582. doi: 10.1093/infdis/129.5.577. [DOI] [PubMed] [Google Scholar]
  28. Short E. C., Kurtz H. J. Properties of the Hemolytic Activities of Escherichia coli. Infect Immun. 1971 May;3(5):678–687. doi: 10.1128/iai.3.5.678-687.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Silva M. L., Maas W. K., Gyles C. L. Isolation and characterization of enterotoxin-deficient mutants of Escherichia coli. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1384–1388. doi: 10.1073/pnas.75.3.1384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith H. W., Halls S. The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production. J Gen Microbiol. 1967 Apr;47(1):153–161. doi: 10.1099/00221287-47-1-153. [DOI] [PubMed] [Google Scholar]
  31. Smith H. W., Linggood M. A. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. J Med Microbiol. 1972 May;5(2):243–250. doi: 10.1099/00222615-5-2-243. [DOI] [PubMed] [Google Scholar]
  32. So M., Boyer H. W., Betlach M., Falkow S. Molecular cloning of an Escherichia coli plasmid determinant than encodes for the production of heat-stable enterotoxin. J Bacteriol. 1976 Oct;128(1):463–472. doi: 10.1128/jb.128.1.463-472.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. So M., Heffron F., McCarthy B. J. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979 Feb 8;277(5696):453–456. doi: 10.1038/277453a0. [DOI] [PubMed] [Google Scholar]
  34. Wachsmuth I. K., Falkow S., Ryder R. W. Plasmid-mediated properties of a heat-stable enterotoxin-producing Escherichia coli associated with infantile diarrhea. Infect Immun. 1976 Aug;14(2):403–407. doi: 10.1128/iai.14.2.403-407.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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