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. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980

Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test.

L Essers, K Radebold
PMCID: PMC273662  PMID: 7024295

Abstract

A latex slide agglutination test detecting clumping factor and protein A simultaneously is recommended for rapid and reliable routine identification of Staphylococcus aureus. Strains (836) of staphylococci isolated from clinical specimens were examined, all S. aureus strains identified by conventional methods were correctly differentiated by the latex test, and no false-positive results occurred with other staphylococci. The reagent is easy to prepare since plasma is the coating material.

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Selected References

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  1. Barry A. L., Lachica R. V., Atchison F. W. Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests. Appl Microbiol. 1973 Mar;25(3):496–497. doi: 10.1128/am.25.3.496-497.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boothby J., Genigeorgis C., Fanelli M. J. Tandem coagulase/thermonuclease agar method for the detection of Staphylococcus aureus. Appl Environ Microbiol. 1979 Feb;37(2):298–302. doi: 10.1128/aem.37.2.298-302.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brun Y., Fleurette J., Forey F. Micromethod for biochemical identification of coagulase-negative staphylococci. J Clin Microbiol. 1978 Nov;8(5):503–508. doi: 10.1128/jcm.8.5.503-508.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Essers L., Radebold K. Protein-A-Hämagglutinationstest, eine zurverlässige Methode zur schnellen Identifizierung von S. aureus. Zentralbl Bakteriol A. 1980;247(2):170–176. [PubMed] [Google Scholar]
  5. Flandrois J. P., Fleurette J., Eyraud F. Détection de la protéine A de Staphylococcus aureus par hémagglutination conditionnée. Ann Biol Clin (Paris) 1975;33(5):365–368. [PubMed] [Google Scholar]
  6. Flandrois J. P., Fleurette J., Thome M. F. Facteurs influencant la production de protéine A par des souches humaines de Staphylococcus aureus. Ann Microbiol (Paris) 1978 Jul;129B(1):57–62. [PubMed] [Google Scholar]
  7. Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
  9. Maxim P. E., Mathews H. L., Mengoli H. F. Single-tube mixed agglutination test for the detection of staphylococcal protein A. J Clin Microbiol. 1976 Nov;4(5):418–422. doi: 10.1128/jcm.4.5.418-422.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Menzies R. E. Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus. J Clin Pathol. 1977 Jul;30(7):606–608. doi: 10.1136/jcp.30.7.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rayman M. K., Park C. E., Philpott J., Todd E. C. Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):451–454. doi: 10.1128/am.29.4.451-454.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SINGER J. M., PLOTZ C. M., GOLDBERG R. THE DETECTION OF ANTI-GLOBULIN FACTORS UTILIZING PRE-COATED LATEX ARTICLES. Arthritis Rheum. 1965 Apr;8:194–202. doi: 10.1002/art.1780080203. [DOI] [PubMed] [Google Scholar]
  13. Sperber W. H., Tatini S. R. Interpretation of the tube coagulase test for identification of Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):502–505. doi: 10.1128/am.29.4.502-505.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. VOGELSANG T. M., WORMNES A., OSTERVOLD B. Correlation between staphylococcal phage groups and some staphylococcal enzymes demonstrated by simple methods. Acta Pathol Microbiol Scand. 1962;54:218–224. doi: 10.1111/j.1699-0463.1962.tb01239.x. [DOI] [PubMed] [Google Scholar]
  15. Wegrzynowicz Z., Heczko P. B., Jeljaszewicz J., Neugebauer M., Pulverer G. Pseudocoagulase activity of staphylococci. J Clin Microbiol. 1979 Jan;9(1):15–19. doi: 10.1128/jcm.9.1.15-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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