Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Nov;12(5):704–708. doi: 10.1128/jcm.12.5.704-708.1980

Visual Reading of Enzyme Immunofluorescence Assays for Human Cytomegalovirus Antibodies

Bagher Forghani 1, Juanita Dennis 1, Nathalie J Schmidt 1
PMCID: PMC273676  PMID: 6268655

Abstract

Enzyme immunofluorescence assays for cytomegalovirus antibodies could be read satisfactorily using a light box with ultraviolet illumination. Higher antibody titers were obtained with a fluorogenic substrate than with a color-producing substrate.

Full text

PDF
704

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  2. Fernley H. N., Walker P. G. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochem J. 1965 Oct;97(1):95–103. doi: 10.1042/bj0970095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forghani B., Schmidt N. J. Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies. J Clin Microbiol. 1979 Jun;9(6):657–664. doi: 10.1128/jcm.9.6.657-664.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forghani B., Schmidt N. J., Dennis J. Antibody assays for varicella-zoster virus: comparison of enzyme immunoassay with neutralization, immune adherence hemagglutination, and complement fixation. J Clin Microbiol. 1978 Nov;8(5):545–552. doi: 10.1128/jcm.8.5.545-552.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forghani B., Schmidt N. J., Lennette E. H. Antisera to human cytomegalovirus produced in hamsters: reactivity in radioimmunoassay and other antibody assay systems. Infect Immun. 1976 Nov;14(5):1184–1190. doi: 10.1128/iai.14.5.1184-1190.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Forghani B., Schmidt N. J., Lennette E. H. Radioimmunoassay of measles virus antigen and antibody in SSPE brain tissue. Proc Soc Exp Biol Med. 1978 Feb;157(2):268–272. doi: 10.3181/00379727-157-40035. [DOI] [PubMed] [Google Scholar]
  7. Kato K., Hamaguchi Y., Fukui H., Ishikawa E. Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay. Eur J Biochem. 1976 Feb 16;62(2):285–292. doi: 10.1111/j.1432-1033.1976.tb10159.x. [DOI] [PubMed] [Google Scholar]
  8. Yolken R. H., Stopa P. J. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus. J Clin Microbiol. 1979 Sep;10(3):317–321. doi: 10.1128/jcm.10.3.317-321.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES