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. 1981 Mar;13(3):427–432. doi: 10.1128/jcm.13.3.427-432.1981

Amino acid requirements of a Chlamydia trachomatis genital strain in McCoy cell cultures.

P Karayiannis, D Hobson
PMCID: PMC273808  PMID: 7240385

Abstract

Amino acid requirements of a Chlamydia trachomatis were defined in McCoy cell monolayer cultures, depleted of their nutrient reserves by maintenance in Earle balanced salt solution for at least 2 days, and then treated at infection with 1 microgram of cycloheximide per ml, an inhibitor of host cell protein and deoxyribonucleic acid synthesis. Serum-free Waymouth MB 752/1 medium lacking one amino acid or a group of related amino acids was added to depleted cultures before infection. Eight of the amino acids normally present in MB 752/1 were essential for chlamydial growth, six were advantageous for growth, and five were nonessential.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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