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. 1981 Mar;13(3):463–467. doi: 10.1128/jcm.13.3.463-467.1981

Inhibition of Neisseria gonorrhoeae by sodium polyanetholesulfonate.

J L Staneck, S Vincent
PMCID: PMC273815  PMID: 6263942

Abstract

Sodium polyanetholesulfonate (SPS), in concentrations commonly used in blood culture media, inhibited the growth of a significant number of isolates of Neisseria gonorrhoeae in an agar dilution system. This SPS toxicity, shown to be bactericidal when examined in broth culture, could be reversed by hemoglobin and gelatin. Gelatin in 1% concentration allowed optimal growth of SPS-sensitive isolates in the presence of 0.025% SPS. Of 50 clinical isolates of N. gonorrheae tested under simulated blood cultures conditions with SPS, 16 isolates failed to grow on subculture at days 1, 3, and 10 after inoculation. Recovery was delayed with eight isolates as compared to controls. Early subcultures at 4, 8, and 12 h failed to recover SPS-sensitive isolates, whereas 1% gelatin, added even as late as 8 h after inoculation, reversed the SPS toxicity. The data reported suggest that SPS at concentrations routinely used in blood cultures can delay or prevent isolation of N. gonorrhoeae, but 1% gelatin can eliminate this adverse effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eng J. Effect of sodium polyanethol sulfonate in blood cultures. J Clin Microbiol. 1975 Feb;1(2):119–123. doi: 10.1128/jcm.1.2.119-123.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eng J., Holten E. Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media. J Clin Microbiol. 1977 Jul;6(1):1–3. doi: 10.1128/jcm.6.1.1-3.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eng J., Iveland H. Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis. J Clin Microbiol. 1975 May;1(5):444–447. doi: 10.1128/jcm.1.5.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gelpi A. P. Gonococcal sepsis in college students. J Am Coll Health Assoc. 1974 Dec;23(2):157–161. [PubMed] [Google Scholar]
  5. Wilkins T. D., West S. E. Medium-dependent inhibition of Peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media. J Clin Microbiol. 1976 Apr;3(4):393–396. doi: 10.1128/jcm.3.4.393-396.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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