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. 1981 Apr;13(4):646–651. doi: 10.1128/jcm.13.4.646-651.1981

Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica.

T M Lin, S P Halbert, C T Chiu, R Zarco
PMCID: PMC273852  PMID: 6262370

Abstract

A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-linked immunosorbent assay is rapid (1 h 15 min, total incubation time), and the reported values are referenced to a positive control so that they correlate with levels of antibody sufficient to be detected by the gel diffusion methods. The enzyme-linked immunosorbent assay is highly reproducible, specific, and sensitive; it can be used qualitatively or quantitatively.

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Selected References

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