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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 May;13(5):882–884. doi: 10.1128/jcm.13.5.882-884.1981

Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques for detection of intestinal parasites.

A L Truant, S H Elliott, M T Kelly, J H Smith
PMCID: PMC273909  PMID: 7240400

Abstract

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered. The Formalin-ether and Formalin-ethyl acetate sedimentation procedures gave identical results for the detection of cysts, ova, and larvae, and these methods offered an advantage over the flotation procedure for the detection of selected ova. However, the zinc sulfate procedure was more effective for the detection of protozoan cysts, Hymenolepis nana, and hookworm eggs. The results indicate that the Formalin-ethyl acetate procedure provides a suitable alternative to the Formalin-ether method, and they demonstrate the value of using both flotation and sedimentation procedures in the analysis of fecal specimens for parasites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett M. S., Harper K., Smith N., Verbanac P., Smith J. W. Comparative evaluation of a modified zinc sulfate flotation technique. J Clin Microbiol. 1978 Jun;7(6):524–528. doi: 10.1128/jcm.7.6.524-528.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Young K. H., Bullock S. L., Melvin D. M., Spruill C. L. Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol. 1979 Dec;10(6):852–853. doi: 10.1128/jcm.10.6.852-853.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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