Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 May;13(5):963–968. doi: 10.1128/jcm.13.5.963-968.1981

Use of New York City medium for improved recovery of Neisseria gonorrhoeae from clinical specimens.

P A Granato, C Schneible-Smith, L B Weiner
PMCID: PMC273924  PMID: 6787078

Abstract

New York City (NYC) and Martin-Lewis (ML) media were evaluated comparatively for their ability to support the growth of Neisseria gonorrhoeae from clinical specimens. A total of 1,010 urethral, cervical, pharyngeal, and rectal specimens were collected from walk-in patients attending a clinic for sexually transmitted diseases. A total of 187 and 165 isolates of gonococci were cultivated on NYC and ML media, respectively, with 161 of these isolates being recovered on both media. Overall, the use of NYC medium resulted in a 13.3% increased recovery rate of gonococci. When gonococci were recovered on both media from primary isolation, the NYC medium supported a more luxuriant growth and a greater number of colonies, which usually resulted in the detection of positive cultures 1 day sooner than on ML medium. Both media were comparable in their ability to suppress the growth of saprophytic microorganisms. The results of this study demonstrated that the use of NYC medium markedly enhanced the recovery of N. gonorrhoeae from clinical specimens as compared to ML medium.

Full text

PDF
967

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brorson J. E., Holmberg I., Nygren B., Seeberg S. Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory. Br J Vener Dis. 1973 Oct;49(5):452–453. doi: 10.1136/sti.49.5.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen N. C., Hipp S. S., Lawton W. D., Gaafar H. A. Gonogrow, an improved, selective medium for the isolation of Neisseria gonorrhoeae. Health Lab Sci. 1974 Jul;11(3):173–177. [PubMed] [Google Scholar]
  3. Cross R. C., Hoger M. B., Neibaur R., Pasternack B., Brady F. J. VCN--inhibited strains of Neisseria gonorrhoeae. HSMHA Health Rep. 1971 Nov;86(11):990–992. [PMC free article] [PubMed] [Google Scholar]
  4. Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity. Health Lab Sci. 1973 Apr;10(2):55–60. [PubMed] [Google Scholar]
  5. Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC nmedium). 3. Performance as a culture and transport medium without addition of ambient carbon dioxide. Health Lab Sci. 1973 Apr;10(2):61–74. [PubMed] [Google Scholar]
  6. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Health Lab Sci. 1973 Apr;10(2):44–54. [PubMed] [Google Scholar]
  7. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. Field evaluation of New York City medium in the biological environment-CO2 chamber in recovery of Neisseria gonorrhoeae and urogenital mycoplasmas. J Clin Microbiol. 1977 Feb;5(2):137–141. doi: 10.1128/jcm.5.2.137-141.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Faur Y. C., Weisburd M. H., Wilson M. E. Morphologic observations of mycoplasmas and Neisseria gonorrhoeae in associated growth patterns. Am J Clin Pathol. 1975 Jan;63(1):106–116. doi: 10.1093/ajcp/63.3.106. [DOI] [PubMed] [Google Scholar]
  9. Faur Y. C., Weisburd M. H., Wilson M. E. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. Health Lab Sci. 1978 Jan;15(1):22–27. [PubMed] [Google Scholar]
  10. Faur Y. C., Weisburd M. H., Wlson M. E., May P. S. NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas. Appl Microbiol. 1974 Jun;27(6):1041–1045. doi: 10.1128/am.27.6.1041-1045.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GRIFFIN P. J., RIEDER S. V. A study on the growth requirements of Neisseria gonorrhoeae and its clinical application. Yale J Biol Med. 1957 Jun;29(6):613–621. [PMC free article] [PubMed] [Google Scholar]
  12. Martin J. E., Armstrong J. H., Smith P. B. New system for cultivation of Neisseria gonorrhoeae. Appl Microbiol. 1974 Apr;27(4):802–805. doi: 10.1128/am.27.4.802-805.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martin J. E., Jr, Lester A. Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis. HSMHA Health Rep. 1971 Jan;86(1):30–33. [PMC free article] [PubMed] [Google Scholar]
  14. Odegaard K., Solberg O., Lind J., Myhre G., Nyland B. Lincomycin in selective medium for the isolation of Neisseria gonorrhoeae. Acta Pathol Microbiol Scand B. 1975 Jun;83(3):301–304. doi: 10.1111/j.1699-0463.1975.tb00106.x. [DOI] [PubMed] [Google Scholar]
  15. Odegaard K. Trimethoprim for the prevention of overgrowth by swarming Proteus in the cultivation of gonococci. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(4):545–548. [PubMed] [Google Scholar]
  16. Reyn A., Bentzon M. W. Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin. Br J Vener Dis. 1972 Oct;48(5):363–368. doi: 10.1136/sti.48.5.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  18. Riddell R. H., Buck A. C. Trimethoprim as an additional selective agent in media for the isolation of N. gonorrhoeae. J Clin Pathol. 1970 Sep;23(6):481–483. doi: 10.1136/jcp.23.6.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seth A. Use of trimethoprim to prevent overgrowth by Proteus in the cultivation of N. gonorrhoeae. Br J Vener Dis. 1970 Jun;46(3):201–202. doi: 10.1136/sti.46.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Symington D. A. An evaluation of New York City transport medium for the detection of Neisseria gonorrhoeae in clinical specimens. Health Lab Sci. 1975 Apr;12(2):69–75. [PubMed] [Google Scholar]
  21. Symington D. A. Improved transport system for Neisseria gonorrhoeae in clinical specimens. J Clin Microbiol. 1975 Dec;2(6):498–503. doi: 10.1128/jcm.2.6.498-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. THAYER J. D., MARTIN J. E., Jr A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS. Public Health Rep. 1964 Jan;79:49–57. [PMC free article] [PubMed] [Google Scholar]
  23. Thayer J. D., Martin J. E., Jr Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis. Public Health Rep. 1966 Jun;81(6):559–562. [PMC free article] [PubMed] [Google Scholar]
  24. Whitbeck E., Shemonsky N., Levison M. E. Superiority of amphotericin over nystatin in Thayer-Martin medium. Antimicrob Agents Chemother. 1975 May;7(5):658–660. doi: 10.1128/aac.7.5.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Young H. Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium. Br J Vener Dis. 1978 Feb;54(1):36–40. doi: 10.1136/sti.54.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES