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. 1975 Nov;2(5):373–376. doi: 10.1128/jcm.2.5.373-376.1975

Thin-layer chromatographic technique for rapid detection of bacterial phospholipases.

N J Legakis, J Papavassiliou
PMCID: PMC274193  PMID: 811682

Abstract

Silica gel thin-layer chromatography was employed to detect lecithinase activity induced from bacterial resting cell preparations induced from bacterial resting cell preparations incubated at 37 C for 4 h in the presence of purified egg yolk lecithin. Bacillus subtilis, Bacillus cereus, Serratia marcescens, and Pseudomonas aeruginosa hydrolyzed lecithin with the formation of free fatty acids as the sole lipid-soluble product. In none of the Escherichia coli and Citrobacter freundii strains tested could lecithinase activity be detected. Four among eight strains of Enterobacter aerogenes and one among 12 strains of Proteus tested produced negligible amounts of free fatty acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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