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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1976 Jun;3(6):604–608. doi: 10.1128/jcm.3.6.604-608.1976

Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.

G C Saunders, E H Clinard
PMCID: PMC274363  PMID: 820713

Abstract

We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.

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Selected References

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