Abstract
Immunization conditions for the production of mouse immune ascitic fluids to be used for the preparation of rabies fluorescent antibody (FA) conjugate are presented. The use of optimal concentrations of ammonium sulfate for precipitation of gamma globulin resulted in a fraction consisting of 75% gamma globulin and 25% alpha-beta globulins with no detectable albumin. Dialysis labeling of the globulin fration with fluorescein isothiocyanate produced a specific rabies FA conjugate with negligible nonspecific background staining. This procedure represents a simple means of producing rabies FA conjugate.
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Selected References
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- Atanasiu P., Tsiang H., Virat J. Obtention d'IgG anti-nucléocapsides rabiques. Purification et conjugaison à la peroxydase ou à l'isothiocyanate de fluorescéine. Ann Microbiol (Paris) 1974 Jul-Aug;125B(1):85–98. [PubMed] [Google Scholar]
- CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
- GOLDWASSER R. A., KISSLING R. E., CARSKI T. R., HOSTY T. S. Fluorescent antibody staining of rabies virus antigens in the salivary glands of rabid animals. Bull World Health Organ. 1959;20:579–588. [PMC free article] [PubMed] [Google Scholar]
- GOLDWASSER R. A., KISSLING R. E. Fluorescent antibody staining of street and fixed rabies virus antigens. Proc Soc Exp Biol Med. 1958 Jun;98(2):219–223. doi: 10.3181/00379727-98-23996. [DOI] [PubMed] [Google Scholar]
- Hebert G. A. Ammonium sulfate fractionation of sera: mouse, hamster, guinea pig, monkey, chimpanzee, swine, chicken, and cattle. Appl Microbiol. 1974 Feb;27(2):389–393. doi: 10.1128/am.27.2.389-393.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hebert G. A., Pelham P. L., Pittman B. Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl Microbiol. 1973 Jan;25(1):26–36. doi: 10.1128/am.25.1.26-36.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LENNETTE E. H., WOODIE J. D., NAKAMURA K., MAGOFFIN R. L. THE DIAGNOSIS OF RABIES BY FLUORESCENT ANTIBODY METHOD (FRA) EMPLOYING IMMUNE HAMSTER SERUM. Health Lab Sci. 1965 Jan;2:24–34. [PubMed] [Google Scholar]
- Sartorelli A. C., Fischer D. S., Downs W. G. Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. J Immunol. 1966 Apr;96(4):676–682. [PubMed] [Google Scholar]
- Tikasingh E. S., Spence L., Downs W. G. The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. Am J Trop Med Hyg. 1966 Mar;15(2):219–226. doi: 10.4269/ajtmh.1966.15.219. [DOI] [PubMed] [Google Scholar]