Abstract
Two new methods for serological grouping of beta-hemolytic streptococci, the nitrous acid extraction procedure of El Kholy et al. and the slide agglutination method of Christensen et al., were compared with the Lancefield hot-hydrochloric acid extraction method in classifying 92 strains of groups A, B, C, and G. The nitrous acid extraction method was easily performed, specific, and sensitive when highly potent antisera were used. For the Christensen method these highly potent antisera had to be diluted to avoid cross-reactions between groups A and C and groups B and G, respectively. A few strains, most of them group B, could not be grouped by the latter method. Using these three grouping methods, two sets of commercial sera were compared with the more potent sera supplied by R. C. Lancefield. The low antibody content of these commercial sera, especially anti-group B and G sera, contributed to the inferior results obtained in some of the grouping reactions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CURTIS S. N., KRAUSE R. M. ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI. J Exp Med. 1964 Oct 1;120:629–637. doi: 10.1084/jem.120.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dajani A. S. Rapid identification of beta hemolytic streptococci by counterimmunoelectrophoresis. J Immunol. 1973 Jun;110(6):1702–1705. [PubMed] [Google Scholar]
- Ederer G. M., Herrmann M. M., Bruce R., Matsen J. M., Chapman S. S. Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol. 1972 Feb;23(2):285–288. doi: 10.1128/am.23.2.285-288.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harrell W. K., George J. R. Quantitative measurement of precipitating antibodies in streptococcal grouping antisera by the single radial immunodiffusion technique. Appl Microbiol. 1972 Jun;23(6):1047–1052. doi: 10.1128/am.23.6.1047-1052.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KRAUSE R. M. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. IV. ANTIGENIC AND BIOCHEMICAL COMPOSITION OF HEMOLYTIC STREPTOCOCCAL CELL WALLS. Bacteriol Rev. 1963 Dec;27:369–380. doi: 10.1128/br.27.4.369-380.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
- Moody M. D., Siegel A. C., Pittman B., Winter C. C. Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs. Am J Public Health Nations Health. 1963 Jul;53(7):1083–1092. doi: 10.2105/ajph.53.7.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
- Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS R. E. Laboratory diagnosis of streptococcal infections. Bull World Health Organ. 1958;19(1):153–176. [PMC free article] [PubMed] [Google Scholar]
- el Kholy A., Wannamaker L. W., Krause R. M. Simplified extraction procedure for serological grouping of beta-hemolytic streptococci. Appl Microbiol. 1974 Nov;28(5):836–839. doi: 10.1128/am.28.5.836-839.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]