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. 1977 Aug;6(2):128–131. doi: 10.1128/jcm.6.2.128-131.1977

Studies on neutralization of human serum bactericidal activity by sodium amylosulfate.

W H Traub
PMCID: PMC274718  PMID: 330560

Abstract

The synthetic anticoagulant sodium amylosulfate (SAS) at concentrations of 125 to 2,000 microgram/ml failed to completely neutralize the bactericidal activity of 80 and 50% (by volume) fresh human serum. Furthermore, SAS failed to inhibit the alternative pathway of complement activation in 80% (by volume) fresh human serum that had been chelated with 0.01 M magnesium ions plus 0.01 M ethyleneglycol-bis(beta-aminoethylether)-N,N-tetraacetic acid. However, SAS at 250 to 1,000 microgram/ml effectively neutralized the bactericidal activity of 20% (by volume) fresh human serum. Therefore, SAS (at 250 to 1,000 microgram/ml) should be used only in blood samples that have been diluted at least fivefold (less than or equal to 20% [by volume]) in suitable broth media.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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